Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe

Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.

Sommario

Characteristics of "host" and "vector". Fission yeast S.pombe: an attractive host for heterologous protein production.- Constitution and development of expression vectors.- Exploiting the thiamine biosynthetic gene nmt1 for regulated expression of heterologous DNAs in fission yeast.- Examples of heterologous protein production in fission yeast.- Overexpression of proteins with seven transmembrane spanning segments.- Targeting and isolationof tagged membrane proteins.- Expression of human cytochrome P450 form in S.pombe.- Human papillomavirus capsid protein-pREP in S.pombe.

Dettagli sul prodotto

• Con la collaborazione di: Yuk Giga-Hama (Editore), Yuko Giga-Hama (Editore), Kumagai (Editore), Kumagai (Editore), Hiromichi Kumagai (Editore)
• Editore: Springer, Berlin
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 03.02.2006
• EAN: 9783540632702
• ISBN: 978-3-540-63270-2
• Pagine: 179
• Peso: 450 g
• Illustrazioni: XVII, 179 p. 46 illus., 4 illus. in color.
• Serie: Biotechnology Intelligence Unit; Biotechnology Intelligence Unit
• Categoria: Scienze naturali, medicina, informatica, tecnica > Biologia > Tematiche generali, enciclopedie