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Informationen zum Autor Professor Haroun N. Shah is Head of Molecular Identification Services Unit at the Centre for Infections, Health Protection Agency, London. The Centre for Infections provides infectious disease surveillance and microbial identification services, co-ordinating the investigation and cause of national and uncommon outbreaks of diseases. Haroun holds several chairs at various universities and spent 25 years in academic life at the University of London. Professor Saheer E. Gharbia is Head of the Applied and Functional Genomics Unit at the HPA Centre for Infections. She has a PhD in molecular Genetics and Biochemistry, and has held various academic positions at the University of London and McGill University, Canada. Klappentext Die neuesten Fortschritte in der Proteomik, die zum großen Teil durch die Entwicklungen in der Massenspektronomie vorangetrieben werden, zeigen immer wieder die Komplexität und Vielfalt pathogener Mechanismen in Mikroben als Verursacher und Verstärker infektiöser Erkrankungen. Eine neue Ära in der medizinischen Mikrobiologie erfordert daher einen schnellen Wechsel der heutigen Verfahren hin zu hoch leistungsfähigen Analysesystemen für die Diagnose mikrobieller Pathogene. Dieses Buch deckt die ganze Bandbreite mikrobiologischer Anwendungen der Proteomik und Massenspektronomie ab. In sechs Kapiteln werden die Abläufe dargestellt, mit denen die meisten mikrobiologischen Labors das Thema angehen: Übergang, Werkzeuge, Vorbereitung, Profiling-by-Pattern, Zielproteine und Datenanalyse. Zusammenfassung New advances in proteomics, driven largely by developments in massspectrometry, continue to reveal the complexity and diversity ofpathogenic mechanisms among microbes that underpin infectiousdiseases. Inhaltsverzeichnis Preface List of contributors Microbial Characterisation; the Transition from Conventional Methods to Proteomics. 1) CHANGING CONCEPTS IN THE CHARACTERISATION OF MICROBES AND THE INFLUENCE OF MASS SPECTROMETRY Haroun Shah et al 1.1 Background and early attempts to use mass spectrometry on microbes. 1.2 Characterisation of microorganisms by MALDI-TOF mass spectrometry; from initial ideas to the development of the first comprehensive database. 1.3 Characterisation of microorganisms from their intracellular/membrane bound protein profiles using affinity capture with particular reference to SELDI-TOF-MS. 1.4 Comparative analysis of proteomes of diverse strains within a species; use of 2-d fluorescence difference gel electrophoresis (dige). 1.5 Searching for low abundant and low molecular weight proteins and peptides using nanoparticles as a selective and concentration probes for MALDI-TOF-MS analysis. 2) MICROBIAL PHYLOGENY AND EVOLUTION BASED ON PROTEIN SEQUENCES (THE CHANGE FROM TARGETED GENES TO PROTEINS) Radhey Gupta 2.1 Abstract 2.2 Microbial phylogeny: overview and key unresolved issues 2.3 New protein-based molecular markers for systematic and evolutionary studies 2.4 Molecular markers elucidating the evolutionary relationships among alpha (a)-proteobacteria 2.5 Molecular markers for the bacteroidetes-chlorobi phyla 2.6 Branching order and interrelationships among bacterial phyla 2.7 Importance of protein markers for discovering unique properties for different groups of bacteria 2.8 Concluding remarks 2.9 Acknowledgements 2.10 References 2: PROTEOMICS TOOLS AND BIOMARKER DISCOVERY. 3) OVERVIEW OF THE PROTEOMIC TOOLS AND IT LINKS TO GENOMICS Raju Misra. 3.1 Protein identification 3.2 Peptide Mass Fingerprint (PMF) 3.3 Peptide Fragment Fingerprint (PFF) 3.4 Peptide sequencing 3.5 False discovery rates (FDR) 3.6 Validating protein identifications 3.7 Reference Database 3.8 Data storage 3.9 Biomarker discovery 3.10 Integrating genomics with proteomics 3.11 Reference List 4) HIGH THROUGHPUT BIOMARKER DISCOVERY IN MICROORGANISMS Ming Fang 4.1 MALDI vs E...
