Ulteriori informazioni
Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.
Sommario
to Patch Clamping.- A Practical Guide to Patch Clamping.- Tight-Seal Whole-Cell Recording.- Guide to Data Acquisition and Analysis.- Methods.- Electronic Design of the Patch Clamp.- Low-Noise Recording.- Voltage Offsets in Patch-Clamp Experiments.- Techniques for Membrane Capacitance Measurements.- Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices.- Patch Clamp and Calcium Imaging in Brain Slices.- Fast Application of Agonists to Isolated Membrane Patches.- Electrochemical Detection of Secretion from Single Cells.- Technical Approaches to Studying Specific Properties of Ion Channels in Plants.- The Giant Membrane Patch.- A Fast Pressure-Clamp Technique for Studying Mechanogated Channels.- Electrophysiological Recordings from Xenopus Oocytes.- Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.- Force Microscopy on Membrane Patches.- Analysis.- The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms.- Fitting and Statistical Analysis of Single-Channel Records.- A Q-Matrix Cookbook.- "Classics".- Geometric Parameters of Pipettes and Membrane Patches.- Conformational Transitions of Ionic Channels.
Riassunto
Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; atomic force microscopy of cells and membranes attached to glass pipettes;
Relazione
From reviews of the First Edition:
"A landmark in the field of membrane biophysics." (Science)
"A first-class laboratory guide...an indispensable reference." (Neuroscience)
