Autophagy in Mammalian Systems

Klappentext Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal house-cleaning in the cell is called autophagy literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 in yeast. These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth. In this volume, a robust slate of methods for conducting research are presented, establishing a consensus of appropriate criteria for monitoring autophagy. Along with with Autophagy: Lower Eukaryotes, this volume marks the seminal collection of methods in the burgeoning field of autophagy. Zusammenfassung Particularly in times of stress! like starvation and disease! higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. This book presents a slate of methods for conducting research! establishing a consensus of appropriate criteria for monitoring autophagy. Inhaltsverzeichnis Chapter 1 LC3-based assay for monitoring autophagy Shunsuke Kimura, Naonobu Fujita, Takeshi Noda and Tamotsu Yoshimori Chapter 2 Analysis of autophagy using GFP-LC3 transgenic mice Noboru Mizushima Chapter 3 Photoactivatable GFP Jennifer Lippincott-Schwartz and Dale Hailey Chapter 4 Assaying for autophagic protein degradation Fred Meijer Chapter 5 Sequestration assays for mammalian autophagy Per O. Seglen Chapter 6 Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment Maria Isabel Colombo Chapter 7 The GST-BHMT assay and related assays for autophagy Carol A. Mercer and Patrick B. Dennis Chapter 8 Redox and autophagy-ROS as an indicator for autophagic activity Ruth Scherz-Shouval and Zvulun Elazar Chapter 9 FACS analysis of autophagy Elena Shvets, Ephraim Fass and Zvulun Elazar Chapter 10 Electron microscopy in mammals Päivi Ylä-Anttila and Eeva-Liisa Eskelinen Chapter 11 Monitoring mTOR activity Ken Inoki Chapter 12 Using p62 as a marker for autophagy. Terje Johansen Chapter 13 Cytosolic LC3 ratio as a quantitative index of macroautophagy Motoni Kadowaki and Md. Razaul Karim Chapter 14 Pexophagy in mammalian cells Junji Ezaki,Takashi Ueno, Eiki Kominami and Masaaki Komatsu Chapter 15 Mitophagy in mammalian cells. Ji Zhang and Paul A. Ney Chapter 16 Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy Tassula Proikas-Cezanne and Simon G. Pfisterer Chapter 17 Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers Sharon Tooze and Minoo Razi Chapter 18 Nanotechnology assays for autophagy Oleksandr Seleverstov Chapter 19 Photoconvertible KAEDA-LC3 for monitoring autophagic flux Christoph Goemans and Aviva Tolkovsky Chapter 20 Methods to monitor Chaperone-mediated autophagy Ana Maria Cuervo and Susmita Kaushik Chapter 21 Autophagy in response to Salmonella enterica serovar Typhimurium infection Cheryl Birmingham and John Brumell Chapter 22 Monitoring autophagy during Mycobacterium tuberculosis infection Vojo Deretic Chapter 23 Shigella, Listeria and Streptococcus induction of autophagy Michinaga Ogawa,Yuko Yoshikawa, Ichiro Nakagawa, Chihiro Sasakawa, and Trinad Chakraborty Chapter 24 Measuring the impact of infection on autophagy in macrophages Jean-Francois Dubuisson, Brenda By...

Sommario

Chapter 1
LC3-based assay for monitoring autophagy
Shunsuke Kimura, Naonobu Fujita, Takeshi Noda and Tamotsu Yoshimori

Chapter 2
Analysis of autophagy using GFP-LC3 transgenic mice
Noboru Mizushima

Chapter 3
Photoactivatable GFP
Jennifer Lippincott-Schwartz and Dale Hailey

Chapter 4
Assaying for autophagic protein degradation
Fred Meijer

Chapter 5
Sequestration assays for mammalian autophagy
Per O. Seglen

Chapter 6
Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment
Maria Isabel Colombo

Chapter 7
The GST-BHMT assay and related assays for autophagy
Carol A. Mercer and Patrick B. Dennis

Chapter 8
Redox and autophagy-ROS as an indicator for autophagic activity
Ruth Scherz-Shouval and Zvulun Elazar

Chapter 9
FACS analysis of autophagy
Elena Shvets, Ephraim Fass and Zvulun Elazar

Chapter 10
Electron microscopy in mammals
Päivi Ylä-Anttila and Eeva-Liisa Eskelinen

Chapter 11
Monitoring mTOR activity
Ken Inoki

Chapter 12
Using p62 as a marker for autophagy.
Terje Johansen

Chapter 13
Cytosolic LC3 ratio as a quantitative index of macroautophagy
Motoni Kadowaki and Md. Razaul Karim

Chapter 14
Pexophagy in mammalian cells
Junji Ezaki,Takashi Ueno, Eiki Kominami and Masaaki Komatsu

Chapter 15
Mitophagy in mammalian cells.
Ji Zhang and Paul A. Ney

Chapter 16
Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy
Tassula Proikas-Cezanne and Simon G. Pfisterer

Chapter 17
Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers
Sharon Tooze and Minoo Razi

Chapter 18
Nanotechnology assays for autophagy
Oleksandr Seleverstov

Chapter 19
Photoconvertible KAEDA-LC3 for monitoring autophagic flux
Christoph Goemans and Aviva Tolkovsky

Chapter 20
Methods to monitor Chaperone-mediated autophagy
Ana Maria Cuervo and Susmita Kaushik

Chapter 21
Autophagy in response to Salmonella enterica serovar Typhimurium infection
Cheryl Birmingham and John Brumell

Chapter 22
Monitoring autophagy during Mycobacterium tuberculosis infection
Vojo Deretic

Chapter 23
Shigella, Listeria and Streptococcus induction of autophagy
Michinaga Ogawa,Yuko Yoshikawa, Ichiro Nakagawa,
Chihiro Sasakawa, and Trinad Chakraborty

Chapter 24
Measuring the impact of infection on autophagy in macrophages
Jean-Francois Dubuisson, Brenda Byrne and Michele Swanson

Dettagli sul prodotto

• Autori: Daniel J. Klionsky
• Con la collaborazione di: Daniel Klionsky (Editore), Daniel (Life Sciences Institute Klionsky (Editore), Daniel J. Klionsky (Editore)
• Editore: ELSEVIER SCIENCE BV
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 05.03.2009
• EAN: 9780123745477
• ISBN: 978-0-12-374547-7
• Pagine: 560
• Serie: Methods in Enzymology; Methods in Enzymology
• Categorie: Scienze naturali, medicina, informatica, tecnica > Fisica, astronomia > Altro; SCIENCE / Life Sciences / Zoology / General, SCIENCE / Life Sciences / Zoology / Mammals, Zoology and animal sciences, Animal Pathology & Diseases, Zoology: Mammals, Zoology: mammals (mammalogy)