Ulteriori informazioni
Informationen zum Autor Editor: Dr Kevin Kavanagh , Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland Klappentext Medical Mycology: cellular and Molecular techniques is a clear and concise overview of the subject that details the techniques essential for ongoing research in the area. Drawing together contributions from both scientists and clinicians working in the field, the text will provide a valuable perspective on the applicability of specific techniques to patient care.A wide range of molecular, immunological and cytological techniques are discussed throughout, with the inclusion of protocol section in each chapter designed to provide both a background a up-to-date account of the applications of each procedure. Every technique is fully referenced and illustrations are provided where required to enhance student understanding.* comprehensive introduction to the key techniques critical to the study of medical mycology* clear explanation of how each technique is applied in the lab* contributions from internationally recognised experts in the field* outlines the background to many techniques required for the successful completion of a research projectAn invaluable reference for students of microbiology, biochemistry and molecular biology as well as postgraduates and researchers in the field of medical mycology looking for an up-to-date overview of the latest laboratory techniques. Zusammenfassung In aller Ausführlichkeit stellt Ihnen dieser Band die medizinische Mykologioe vor. Führende Wissenschaftler auf diesem Gebiet erläutern Grundlagen und wichtige experimentelle Verfahren. Inhaltsverzeichnis Preface xiii List of Contributors xv 1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry 1 Malcolm D. Richardson, Riina Rautemaa and Jarkko Hietanen 1.1 Introduction 1 1.2 Specificity of monoclonal antibody 3H8 for C. albicans 3 Protocol 1.1 Testing of specificity of monoclonal antibody 3H8 4 1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms 6 Protocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms 6 1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease 7 Protocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue 8 1.5 References 11 2 Transmission Electron Microscopy of Pathogenic Fungi 13 Guy Tronchin and Jean-Philippe Bouchara 2.1 Introduction 13 2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis 16 Protocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis 17 2.3 Identification of the different compartments of the secretory pathway in yeasts 18 Protocol 2.2 Identification of the different compartments of the secretory pathway in yeasts 19 2.4 Cytochemical localization of acid phosphatase in yeasts 20 Protocol 2.3 Cytochemical localization of acid phosphatase in yeasts 21 2.5 Detection of anionic sites 23 Protocol 2.4 Detection of anionic sites 23 2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 25 Protocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 26 2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wall 28 Protocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall 29 2.8 Detection of glycoconjugates by the lectin-gold technique 30 Protocol 2.7 Detection of glycoconjugates by the lectin-gold technique 31 2.9 Immunogold detection of antigens ...
