Macromolecular Crystallography Protocols, Volume 2 - Structure Determination

In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures.

Sommario

Cryocrystallography of Macromolecules.- Determination of Reaction Intermediate Structures in Heme Proteins.- Annealing Macromolecular Crystals.- First Analysis of Macromolecular Crystals.- X-Ray Data Collection From Macromolecular Crystals.- Characterizing a Crystal From an Initial Native Dataset.- Molecular Replacement.- Phase Determination Using Halide Ions.- The Same But Different.- Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement With Anomalous Scattering Data Using Shake-and-Bake.- Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments.- Automated Structure Solution With autoSHARP.- to Macromolecular Refinement.- Quality Control and Validation.- Crystallographic Software.

Riassunto

In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures.

Dettagli sul prodotto

• Con la collaborazione di: Sylvi Doublie (Editore), Sylvie Doublie (Editore)
• Editore: Springer, Berlin
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 17.04.2009
• EAN: 9781588299024
• ISBN: 978-1-58829-902-4
• Pagine: 284
• Peso: 607 g
• Illustrazioni: XII, 284 p. 70 illus.
• Serie: Methods in Molecular Biology; Methods in Molecular Biology
• Categorie: Scienze naturali, medicina, informatica, tecnica > Biologia > Microbiologia; Biochemie, Molekularbiologie, Zytologie, C, Biowissenschaften, allgemein, ZELLBIOLOGIE, Cytologie, Zelle (biologisch) / Zellbiologie, Biologie / Zellbiologie, Zelle (biologisch) / Zytologie, SCIENCE / Life Sciences / Biochemistry, Mikrobiologie - Mikroorganismus, Biologie / Mikrobiologie, Mikrobiologie (nicht-medizinisch), Biologie / Molekularbiologie, Kristallografie, Kristall - Kristallisation - Kristallografie, SCIENCE / Life Sciences / Cell Biology, SCIENCE / Physics / Crystallography, biochemistry, proteins, Biomedical and Life Sciences, Cell Biology