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This detailed volume explores methods and protocols employed to investigate the multifaceted processes involved in plant stress memory. Opening with foundational methods for studying thermomemory in Arabidopsis thaliana, the book continues with techniques for studying memory mechanisms in other plant species, memory of biotic stressors, and the critical role of signaling molecules, such as jasmonic acid and salicylic acid. A key focus of this book is the investigation of DNA methylation and other epigenetic modifications that play a central role in the transmission of stress memory across generations, and epigenetic and transcriptomic approaches plus cutting-edge techniques such as RNA-Seq, CRISPR/Cas9-based gene editing, and proteomic analyses are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Stress Memory: Methods and Protocols serves as an invaluable resource for researchers, students, and practitioners working to unravel the mysteries of plant stress memory and apply this knowledge to address global challenges in agriculture and environmental sustainability.
Sommario
Thermomemory Assay of Arabidopsis Plants.- Assays to Study Plant Response to High Temperature in Arabidopsis: Evidence in Glutathione Biosynthesis Mutants.- Application of UV-A Radiation to Induce Tolerance to Biotic and Abiotic Stress in Horticultural Crops.- Assessment of Transgenerational Plasticity of Salinity Tolerance in Rice.- Studying DNA Methylation for Transgenerational Inheritance.- Single-Base Resolution DNA Methylation Analysis Through Targeted Locus-Specific Bisulfite Sequencing in Plants.- Targeted Bisulfite Sequencing and DNA Methylation Analysis in Rice Endosperm and Other Tissues.- Immunofluorescence Staining and Microscopic Imaging of Plant Nuclei for Epigenetic Modifications.- iTRAQ-Based Proteomic Approach to Study Stress Memory Responses in Plants.- Mass Spectrometry-Based Methods for Plant Metabolite Profiling.- Post-Translational Modifications in Proteins Under Stress and Memory.- RNA-Seq Analysis in Plant Stress Memory: A Bioinformatics Protocol.- Target-Specific Single Guide RNA (sgRNA) Design and In Vitro Validation of Target-Specific sgRNAs for CRISPR/Cas9-Mediated Editing of a Plant Stress Memory-Associated Gene.- Studying Heat-Stress Memory in Red Algae.- Method to Study Induced Systemic Resistance Against Bacterial Pathogens in Orchids Primed by Root Colonization of Mycorrhizal Fungi.- Priming Seeds and Plants with Microbial Inoculants to Improve Growth and Stress Memory.- Quantification of Jasmonic Acid and Salicylic Acid in Plant-Insect Interactions.- Quantifying Arbuscular Mycorrhizal Fungal Colonization via Anthocyanin Pigmentation in Medicago truncatula Roots.- Insect Herbivory Simulation and Insect Bioassays to Study Plant Stress Memory Response.
Riassunto
This detailed volume explores methods and protocols employed to investigate the multifaceted processes involved in plant stress memory. Opening with foundational methods for studying thermomemory in Arabidopsis thaliana, the book continues with techniques for studying memory mechanisms in other plant species, memory of biotic stressors, and the critical role of signaling molecules, such as jasmonic acid and salicylic acid. A key focus of this book is the investigation of DNA methylation and other epigenetic modifications that play a central role in the transmission of stress memory across generations, and epigenetic and transcriptomic approaches plus cutting-edge techniques such as RNA-Seq, CRISPR/Cas9-based gene editing, and proteomic analyses are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Stress Memory: Methods and Protocols serves as an invaluable resource for researchers, students, and practitioners working to unravel the mysteries of plant stress memory and apply this knowledge to address global challenges in agriculture and environmental sustainability.
