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This detailed volume presents protocols centered on the sensing and signaling of ethylene, a plant hormone with a vital role in fruit ripening, seed germination, stress adaptation, and a wide array of developmental processes. Beginning with a section on ethylene detection and quantification techniques, including gas chromatography and analysis during hypoxia and pathogen interactions, the book continues by examining the role of ethylene in post-harvest biology and fruit ripening, and associated regulatory mechanisms, including promoter characterization and protein-protein interactions. Further sections explore bioinformatics workflows for unraveling mechanistic roles of ethylene at the cellular and whole-plant levels, as well as the regulatory capacity of ethylene in plant developmental processes, root system modifications, and adaptation to diverse biotic and abiotic stressors. Written for the highly successful Methods in Molecular Biology series, chapters offer readers a hands-on approach to investigate the myriad roles that ethylene plays in plant growth and development.
Authoritative and practical, Ethylene Sensing and Signaling serves as a key resource for scientists seeking to employ reliable methodologies in understanding the central function of ethylene in plant biology.
Sommario
Estimation of 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Deaminase Enzyme in Bacteria.- Method for Measurement of Ethylene during Hypoxia in Rice Plants.- Method for Measurement of Ethylene during Pathogen Infection in Arabidopsis.- Determination of the Effect of Ethylene Treatment on the Ripening Process, Weight Loss, Free Humidity, Colorimetry, and Drying Kinetics of Solanum lycopersicum L (Tomato).- Ethylene Estimation of In Vivo Cultured Plants by Gas Chromatography.- Measuring Internal and External Ethylene Concentration in Fruits and Vegetables.- Ethylene-Mediated Regulation of a Ripening Specific N-Glycan Processing Enzyme -D-N-Acetylhexosaminidase.- The Role of Ethylene and Its Antagonists in Advancing Post-Harvest Management of Fruits and Flowers.- Isolation and Characterization of Proximal Promoters Regulating Ethylene-Induced Ripening in Banana.- Deciphering Ethylene Signaling via Protein-Protein Interaction in Tomato Fruit.- Fundamental Techniques to Evaluate the Roles of Ethylene Receptors in Seed Germination under Salt Stress in Arabidopsis thaliana.- GCN2 and EIN2 Pathways Activation Assays in Arabidopsis Under Hypoxia.- The Detection of Ethylene Signaling Using Bioinformatics Tools in Plants.- Luciferase Complementation Imaging Assay (LCI) to Reveal the Interaction between SPL7 and RAN1 Genes during Ethylene Signaling.- Ethylene Signaling in Plant Development and Stress Adaptation.- Mapping of the Ethylene-Specific Root System Architecture (RSA) Traits in Arabidopsis.- Ethylene as a Central Regulator of Seed Germination, Early Seedling Development, and Stress Adaptation.- Role of Ethylene in Plant Tissue Culture.
Riassunto
This detailed volume presents protocols centered on the sensing and signaling of ethylene, a plant hormone with a vital role in fruit ripening, seed germination, stress adaptation, and a wide array of developmental processes. Beginning with a section on ethylene detection and quantification techniques, including gas chromatography and analysis during hypoxia and pathogen interactions, the book continues by examining the role of ethylene in post-harvest biology and fruit ripening, and associated regulatory mechanisms, including promoter characterization and protein-protein interactions. Further sections explore bioinformatics workflows for unraveling mechanistic roles of ethylene at the cellular and whole-plant levels, as well as the regulatory capacity of ethylene in plant developmental processes, root system modifications, and adaptation to diverse biotic and abiotic stressors. Written for the highly successful Methods in Molecular Biology series, chapters offer readers a hands-on approach to investigate the myriad roles that ethylene plays in plant growth and development.
Authoritative and practical, Ethylene Sensing and Signaling serves as a key resource for scientists seeking to employ reliable methodologies in understanding the central function of ethylene in plant biology.
