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Informationen zum Autor Edmond de Hoffmann , Université Catholique de Louvain, Belgium & Ludwig Institute for Cancer Research, Brussels, Belgium. Vincent Stroobant , Ludwig Institute for Cancer Research, Brussels, Belgium. Klappentext The latest edition of a highly successful textbook, Mass Spectrometry, Third Edition provides students with a complete overview of the principles, theories and key applications of modern mass spectrometry. All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analysers and detectors. Tandem mass spectrometry is introduced early on and then developed in more detail in a later chapter. Emphasis is placed throughout the text on optimal utilisation conditions. Various fragmentation patterns are described together with analytical information that derives from the mass spectra. This new edition has been thoroughly revised and updated and has been redesigned to give the book a more contemporary look. As with previous editions it contains numerous examples, references and a series of exercises of increasing difficulty to encourage student understanding. Updates include: Increased coverage of MALDI and ESI, more detailed description of time of flight spectrometers, new material on isotope ratio mass spectrometry, and an expanded range of applications. Mass Spectrometry, Third Edition is an invaluable resource for all undergraduate and postgraduate students using this technique in departments of chemistry, biochemistry, medicine, pharmacology, agriculture, material science and food science. It is also of interest for researchers looking for an overview of the latest techniques and developments. Zusammenfassung The latest edition of a highly successful textbook, Mass Spectrometry, Third Edition provides students with a complete overview of the principles, theories and key applications of modern mass spectrometry. All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analyzers and detectors. Inhaltsverzeichnis Preface xi Introduction 1 Principles 1 Diagram of a Mass Spectrometer 4 History 5 Ion Free Path 10 1 Ion Sources 15 1.1 Electron Ionization 15 1.2 Chemical Ionization 17 1.2.1 Proton transfer 19 1.2.2 Adduct formation 21 1.2.3 Charge-transfer chemical ionization 21 1.2.4 Reagent gas 22 1.2.5 Negative ion formation 25 1.2.6 Desorption chemical ionization 27 1.3 Field Ionization 28 1.4 Fast Atom Bombardment and Liquid Secondary Ion Mass Spectrometry 29 1.5 Field Desorption 31 1.6 Plasma Desorption 32 1.7 Laser Desorption 33 1.8 Matrix-Assisted Laser Desorption Ionization 33 1.8.1 Principle of MALDI 33 1.8.2 Practical considerations 36 1.8.3 Fragmentations 39 1.8.4 Atmospheric pressure matrix-assisted laser desorption ionization 39 1.9 Thermospray 41 1.10 Atmospheric Pressure Ionization 42 1.11 Electrospray 43 1.11.1 Multiply charged ions 46 1.11.2 Electrochemistry and electric field as origins of multiply charged ions 48 1.11.3 Sensitivity to concentration 50 1.11.4 Limitation of ion current from the source by the electrochemical process 51 1.11.5 Practical considerations 54 1.12 Atmospheric Pressure Chemical Ionization 55 1.13 Atmospheric Pressure Photoionization 56 1.14 Atmospheric Pressure Secondary Ion Mass Spectrometry 61 1.14.1 Desorption electrospray ionization 61 1.14.2 Direct analysis in real time 62 1.15 Inorganic Ionization Sources 65 1.15.1 Thermal ionization source 65 1.15.2 Spark source 67 1.15.3 Glow discharge source 68 1.15.4 Inductively coupled plasma source 69 1.15.5 Practical consi...
