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Informationen zum Autor Sharmila Bapat, PHD, is a group leader of a small research group at the National Centre for Cell Science at India's University of Pune. She is an author of the first report on the isolation and identification of stem cells in ovarian cancer. Dr. Bapat organizes workshops on topics such as cell line establishment, immortalization of cultured cells, and stem cell protocols. She is also on the board of editors of the recently launched Journal of Ovarian Research. Klappentext Because the concept and discoveries of cancer stem cells are relatively new, scientists and researchers need an introduction to this dynamic area. Cancer Stem Cells presents a consolidated account of the research done to date and recent progresses in the studies of cancer stem cells. Such a presentation facilitates a better understanding of and draws attention to stem cell and cancer biology - two fields that enhance, move, and evolve into each other continuously. It provides an informative study in designing approaches to apply stem cell principles to cancer biology while offering an overview of the challenges in developing combination stem and cancer biology targets for therapeutics. This book serves as a primer for new researchers in the field of cancer biology. Zusammenfassung Because the concept and discoveries of cancer stem cells are relatively new, scientists and researchers need an introduction to this dynamic area. Cancer Stem Cells presents a consolidated account of the research done to date and recent progresses in the studies of cancer stem cells. Inhaltsverzeichnis Contributors xi Preface xiii 1 Cancer Stem Cells: Similarities and Variations in the Theme of Normal Stem Cells 1 Sharmila Bapat, Anne Collins, Michael Dean, Kenneth Nephew, and Suraiya Rasheed 1.1 Introduction 1 1.2 Stem Cells in the Life of an Organism 2 1.2.1 Stem Cells in Early Development and Fetal Life 3 1.2.2 Stem Cells in the Adult Organism 4 1.3 Cancer Stem Cells 7 1.3.1 Activation of Stem Cells and Cancer 7 1.3.2 Isolation and Identification of Cancer Stem Cells 10 1.3.3 De Novo Generation of a New Organ (Tumor) by Transformed Stem Cells 12 1.4 Self-Renewal and Differentiation in CSCs 13 1.5 CSC Plasticity as Regulated by Intrinsic and Extrinsic Stem Cell Factors 14 1.5.1 Stem Cell Intrinsic Factors: Genetic and Epigenetic Effects 14 1.5.2 Stem Cell Extrinsic Effects: Niche Effects and Microenvironmental Signaling 16 1.6 Conclusions and Future Perspectives 18 References 20 2 Leukemic Stem Cells 27 Sharmila Bapat 2.1 Introduction 27 2.2 Dysregulation of Hematopoiesis in Leukemia 28 2.2.1 Normal Hematopoietic Stem Cell Hierarchies 28 2.2.2 Understanding Aberrant Hierarchies in Leukemia 30 2.2.3 Types of Leukemia 31 2.3 Identification and isolation of Cancer-Initiating Cells in Leukemia 35 2.4 Molecular Regulation of Aberrant Hierarchies 36 2.4.1 Signaling Pathways Deregulated in Leukemia 37 2.4.2 Self-Renewal of Normal and Leukemic Stem Cells 39 2.4.3 Epigenetic Effects 40 2.4.4 MicroRNA in Leukemia Development 42 2.5 Conclusions and Future Perspectives 44 References 45 3 Isolation and Characterization of Breast and Brain Cancer Stem Cells 57 Meera Saxena and Annapoorni Rangarajan 3.1 Introduction 57 3.2 Breast Cancer Stem Cells 58 3.2.1 Mammary Gland Architecture and Cell Types 58 3.2.2 Breast Cancer 59 3.2.3 Identification of Breast Cancer Stem Cells 59 3.2.4 Putative Breast Cancer Stem Cells that Exhibit the CD44+ CD24-/low Lin- Marker Profile 61 3.2.5 ESA+ Subpopulation of CD24-low Lin- Cells Enriched by Tumorigenicity 61 3.2.6 Tumorigenic Breast Cells Displaying Properties of S...
