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This detailed new edition gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. New chapters focus on the latest technologies that have improved our knowledge in this field, such as organoids, machine learning, nanoparticles, and other recent advances. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Cancer Stem Cells: Methods and Protocols, Second Edition provides researchers with the key techniques needed to increase our understanding of this important field of study.
Sommario
Cancer Stem Cells: Current Challenges and Future Perspectives.- Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods.- Isolating Cancer Stem Cells from Solid Tumors.- Surface Markers for the Identification of Cancer Stem Cells.- CD44-Based Detection of CSCs: CD44 Immunodetection by Flow Cytometry.- ALDH Activity Assay: A Method for Cancer Stem Cell (CSC) Identification and Isolation.- In Vitro Tumorigenic Assay: A Tumor Sphere Assay for Cancer Stem Cells.- Multicellular Tumoroids for Investigating Cancer Stem-Like Cells in the Heterogeneous Tumor Microenvironment.- Generation, Expansion, and Biobanking of Gastrointestinal Patient-Derived Organoids from Tumor and Normal Tissues.- Prostate Cancer Organoids for Tumor Modeling and Drug Screening.- Mimicking the Tumor Niche: Methods for Isolation, Culture, and Characterization of Cancer Stem Cells and Multicellular Spheroids.- Detection of Cancer Stem Cells in Normal and Dysplastic/Leukemic Human Blood.- Methods to Study the Role of Mechanical Signals in the Induction of Cancer Stem Cells.- Co-Delivery Polymeric Poly(Lactic-co-Glycolic Acid) (PLGA) Nanoparticles to Target Cancer Stem-Like Cells.- Isolating Circulating Cancer Stem Cells (CCSCs) from Human Whole Blood.- Generation of Cancer Stem Cells by Co-Culture Methods.- Deep Learning of Cancer Stem Cell Morphology.
Riassunto
This detailed new edition gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. New chapters focus on the latest technologies that have improved our knowledge in this field, such as organoids, machine learning, nanoparticles, and other recent advances. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Cancer Stem Cells: Methods and Protocols, Second Edition provides researchers with the key techniques needed to increase our understanding of this important field of study.
