DNA Arrays - Methods and Protocols

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays-from their conception to their ready off-the-shelf availability-for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .

Sommario

DNA Microarrays.- Gel-Immobilized Microarrays of Nucleic Acids and Proteins.- Sequencing by Hybridization Arrays.- Ethical Ramifications of Genetic Analysis Using DNA Arrays.- Photolithographic Synthesis of High-Density Oligonucleotide Arrays.- Automated Genotyping Using the DNA MassArray(TM) Technology.- Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules.- Printing DNA Microarrays Using the Biomek® 2000 Laboratory Automation Workstation.- Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays.- Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays.- DNA Sequencing by Hybridization with Arrays of Samples or Probes.- Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents.- Low-Resolution Typing of HLA-DQA1 Using DNA Microarray.- Gene Expression Analysis on Medium-Density Oligonucleotide Arrays.- Use of Bioinformatics in Arrays.- Confocal Scanning of Genetic Microarrays.- Business Aspects of Biochip Technologies.

Riassunto

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .

Testo aggiuntivo

"DNA Arrays - Methods and Protocols presents microarray technology as an extremely useful tool to better understand modern molecular biology, particularly DNA sequencing, gene expression, genetic mutation and polymorphism. ... The main focus is on a very detailed presentation of the available methods in microarray technology. Experiments are explained carefully and systematic attention is paid to possible variations and particular difficulties that may arise. ...Useful tips are offered: as to how to perform direct comparisons of gene-expression patterns between two tissues or two different experimental condition... this book will surely be viewed as one that represented a stepping stone into development of the technique."- Department of Chemistry, University of Toronto

"DNA Arrays: Methods and Protocols is both a useful introduction to DNA microarray technology as well as a handy source of protocols for specific applications of the technique. The book covers a broad range of topics related to DNA microarrays beginning of DNA arrays to the analysis of the data that is produced....This book offers many practical insights into DNA microarray analysis that should prove to be useful to scientists interested in implementing this technique in their research....the reader should be able to easily follow the progression of the material through the different stages one would need to perform to design a microarray experiment. This book will be of interest for those readers seeking practical information about DNA array methodologies." - Biotech Software & Internet Report

"...of interest to postgraduate students and postdoctoral researchers who have begun work on DNA arrays and need a broader view of the field, together with useful tips for solving practical problems." - Angewente Chemie International

"DNA Arrays-Methods and Protocols presents microarray technology as an extremely useful tool to better understand modern molecular biology, particularly DNA sequencing, gene expression, genetic mutation and polymorphism." -The Analyst

"...a comprehensive guide to DNA microarrays...provides a starting point where one can begin to tackle the vast amount of new literature on the subject." -The National Journal of India

Relazione

"DNA Arrays - Methods and Protocols presents microarray technology as an extremely useful tool to better understand modern molecular biology, particularly DNA sequencing, gene expression, genetic mutation and polymorphism. ... The main focus is on a very detailed presentation of the available methods in microarray technology. Experiments are explained carefully and systematic attention is paid to possible variations and particular difficulties that may arise. ...Useful tips are offered: as to how to perform direct comparisons of gene-expression patterns between two tissues or two different experimental condition... this book will surely be viewed as one that represented a stepping stone into development of the technique."- Department of Chemistry, University of Toronto

"DNA Arrays: Methods and Protocols is both a useful introduction to DNA microarray technology as well as a handy source of protocols for specific applications of the technique. The book covers a broad range of topics related to DNA microarrays beginning of DNA arrays to the analysis of the data that is produced....This book offers many practical insights into DNA microarray analysis that should prove to be useful to scientists interested in implementing this technique in their research....the reader should be able to easily follow the progression of the material through the different stages one would need to perform to design a microarray experiment. This book will be of interest for those readers seeking practical information about DNA array methodologies." - Biotech Software & Internet Report

"...of interest to postgraduate students and postdoctoral researchers who have begun work on DNA arrays and need a broader view of the field, together with useful tips for solving practical problems." - Angewente Chemie International

"DNA Arrays-Methods and Protocols presents microarray technology as an extremely useful tool to better understand modern molecular biology, particularly DNA sequencing, gene expression, genetic mutation and polymorphism." -The Analyst

"...a comprehensive guide to DNA microarrays...provides a starting point where one can begin to tackle the vast amount of new literature on the subject." -The National Journal of India

Dettagli sul prodotto

• Con la collaborazione di: Jan B Rampal (Editore), Jang B Rampal (Editore), Jang B. Rampal (Editore)
• Editore: Springer, Berlin
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 09.07.2009
• EAN: 9780896038226
• ISBN: 978-0-89603-822-6
• Pagine: 268
• Peso: 581 g
• Illustrazioni: XII, 268 p.
• Serie: Methods in Molecular Biology; Methods in Molecular Biology
• Categorie: Scienze naturali, medicina, informatica, tecnica > Biologia > Tematiche generali, enciclopedie; B, Biomedical and Life Sciences, Cell Biology