Mycobacteria Protocols

Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis. In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.
Mycobacteria Protocols contains a unique collection of both established and recently developed techniques that have been optimized and adapted for use with mycobacteria. Offering an eminently practical introduction for all those carrying out basic or applied research in this important class of bacteria, Mycobacteria Protocols is certain to become the new standard reference in the field, one from which even expert researchers will learn many time-saving techniques.

Sommario

Mycobacteria: Bugs and Bugbears
Tanya Parish and Neil G. Stoker
Mycobacteria: General Culture Methodology and Safety Considerations
Bryan W. Allen
Isolation of Genomic DNA from Mycobacteria
John T. Belisle and Michael G. Sonnenberg
Nucleic-Acid Extraction: Plasmid/Cosmid DNA
Bonnie B. Plikaytis and C. Harold King
Pulsed Field Gel Electrophoresis for Mycobacteria
Wolfgang J. Philipp,Stephen Gordon, Amalio Telenti, and Stewart T. Cole
Extraction of RNA from Mycobacteria
Eshwar Mahenthiralingam
Preparation of Cell-Free Extracts from Mycobacteria
Tanya Parish and Paul R. Wheeler
Preparation of Cell-Wall Fractions from Mycobacteria
Gurdyal S. Besra
Using MycDB on the World-Wide Web
Staffan Bergh and Stewart T. Cole
Conjugating DNA into Mycobacteria
Julie-Ann Gavigan and Carlos Martin
Electroporation of Mycobacteria
Tanya Parish and Neil G. Stoker
Mycobacteriophages
Gary J. Sarkis and Graham F. Hatfull
Chemical Mutagenesis of Mycobacteria
Lucy A. Brooks
Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System
Esther Pérez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giguel, and Carlos Martin
Homologous Recombination in Mycobacterium smegmatis: Screening Methods for Detection of Gene Replacement
Robert N. Husson
Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker
Peter Sander and Erik C. Böttger
Gene Replacement in Mycobacterium intracellulare
Britt-Inger Marklund and Richard W. Stokes
Gene Replacement in Mycobacterium bovis BCG
Elizabeth Norman
The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria
Stephen Gordon, Gavin Chung, and Peter Andrew
Green Fluorescent Protein: A Tool for Gene Expression and Cell Biology in Mycobacteria
Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, and V. Deretic
Expression of Genes in Mycobacteria
Peadar - Gaora
Secretion of Mammalian Proteins from Mycobacteria
Peter J. Murray and Richard A. Young
Intracellular Gene Expression: Analysis of RNA from Mycobacteria in Macrophages Using RT-PCR
Philip D. Butcher, Joseph A. Mangan, and Irene M. Monahan
Analysis of Mycobacterial Differential Gene Expression by RAP-PCR
Joseph A. Mangan and Philip D. Butcher
Mycobacterial Speciation
Francis A. Drobniewski and Anne H. C. Uttley
Species Identification of Mycobacteria Using rDNA Sequencing
Philip Kirschner and Erik C. Böttger
Detection of Mycobacterium tuberculosis by a Colorimetric Polymerase Chain Reaction
Stuart M. Wilson
Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping
Henri O. F. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, and Jan D. A. van Embden
Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis
Herre F. Heersma, Kristin Kremer, and Jan D. A. van Embden
Detection of Mutations in Mycobacteria by PCR-SSCP(Single-Strand Conformation Polymorphism)
Amalio Telenti, Nadine Honoré, and Stewart T. Cole
The Use of Luciferase-Reporter Phage for Antibody-Specific Testing of Mycobacteria
Paul F. Riska and William R. Jacobs, Jr
Index

Relazione

"...this book is an essential purchase for an laboratory dealing with mycobacteria and good value for individual researchers." -Microbiology Today "...a unique book...presents excellent description of many of the molecular biological tools that have been developed...an invaluable collection of methodologies for mycobacterial research...the molecular mycobacteriologist will find much of interest in this book...Worth getting." -Journal of Medical Microbiology "...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms...of interest to the epidemiologist is the description of computer analysis of DNA fingerprinting for tuberculosis while the chapter on accessing MycDB, a database dedicated to mycobacterial research, will be of value to all those working in this area."-Molecular Biotechnology "...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms....The detailed technical nature of each chapter provides clear instruction for those wishing to engage in this area of research." - Molecular Biotechnology

Dettagli sul prodotto

• Con la collaborazione di: Tanya Parish (Editore), Neil G. Stoker (Editore)
• Editore: Springer, Berlin
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 12.04.2012
• EAN: 9780896034716
• ISBN: 978-0-89603-471-6
• Pagine: 472
• Dimensioni: 156 mm x 229 mm x 26 mm
• Peso: 2 g
• Illustrazioni: 62 SW-Abb.,
• Serie: Methods in Molecular Biology; Methods in Molecular Biology
• Categoria: Scienze naturali, medicina, informatica, tecnica > Biologia > Microbiologia