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This book provides comprehensive knowledge and a detailed step by step description of experimental protocols for the determination of food intake, body weight changes, and some metabolic markers. Chapters are split into two parts detailing experimental diets, monitor food intake, weight gain, evaluate biological samples, predict physiological changes, evaluate bioavailability of bioactive, anamnesis, measure the metabolic rate, assessing the body composition, assessing glucose homeostasis, and monitoring the metabolomic pathways.
Authoritative and cutting-edge, Basic Protocols in Foods and Nutrition aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
Sommario
Experimental Diets (Normocaloric and Hypercaloric Diets).- Measures of Food Intake, Body Weight Gain, and Energy Efficiency in Mice.- The assessment of glucose homeostasis in rodents: Glucose, Insulin and Pyruvate.- Histopathological Evaluation of Steatohepatitis in Animal Experiments.- Quantification of short-chain fatty acids in feces.- Quantification of non-esterified fatty acids in serum and plasma.- Lipid Peroxidation (TBARS) in biological samples.- Extraction of Bile Acids from Biological Samples and Quantification Using Ultra-High-Performance Liquid Chromatography-Orbitrap Mass Spectrometry.- Glycogen measurement.- Evaluation of the minerals and trace elements in the biological samples.- Microbiome Evaluation.- The GSH colorimetric method as measurement of antioxidant status in serum and rodent tissues.- Guidance for designing a preclinical bioavailability study of bioactive compounds.- Protocols related to Nutritional Anamnesis in Head and Neck Cancer Patients.- Food Diary, Food Frequency Questionnaire, and 24-hour dietary recall.- The Use of Questionnaires to Measure Appetite.- Protocols for the Use of Indirect Calorimetry in Clinical Research.- Anthropometric Assessment Methods for Adults and Older People.- MRI-based Body Composition Analysis.- Functional tests for assessing human beta-cell function and insulin sensitivity.- Maximum oxygen consumption - VO2max laboratory assessment.- Muscle and Fat Biopsy and Metabolomics.
