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This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book's contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the "wellbeing" of cells in various environments.
Sommario
Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness.- The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability.- Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions.- Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability.- Assaying Mitochondrial Function by Multi-Parametric Flow Cytometry.- High-Efficiency Single Cell Electrical Impedance Spectroscopy.- Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis.- Average Rheological Quantities of Cells in Monolayers.- Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays.- Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch.- Assessment of Cell Viability in Electrically Excitable Muscle Cells through Intact Twitch Stimulation.-Evaluating Cellular Viability by iTRAQ Proteomic Profiling.- Quantifying pH in Malaria Using pHluorin and Flow Cytometry.- Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles.- Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro.- Digital Holographic Microscopy to Assess Cell Behavior.- Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells.- Optimization of Cell Viability Assays for Drug Sensitivity Screens.- Cellasys #8: A Microphysiometric Assay for Real-Time Cell Analysis within 24 Hours.- Live Cell Adhesion, Migration, and Invasion Assays.- Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields.- Transwell In Vitro Cell Migration and Invasion Assays.- Live Cell Calcium Imaging of Non-Adherent Cells.- FUCCI Reporter Gene-Based Cell Cycle Analysis.- Cell Viability Assays for 3D Cellular Constructs.- Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken's Chorioallantoic Membrane (CAM Assay).- Assessment of Tissue Viability by Functional Imaging of Membrane Potential.- Multiplexed Viability Assays for High-Throughput Screening of Spheroids of Multiple Sizes.- Tracking Gut Motility in Organ and Cultures.- Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering.
