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Informationen zum Autor Olivier Cochet is the editor of Immunological Techniques Made Easy , published by Wiley. Jean-Luc Teillaud is the editor of Immunological Techniques Made Easy , published by Wiley. Catherine Sautès is the editor of Immunological Techniques Made Easy , published by Wiley. Klappentext Immunological Techniques Made Easy Edited by Olivier Cochet, Biotechnology and Antibody Laboratory, Jean-Luc Teillaud and Catherine Sautès, INSERM Laboratory of Cellular and Clinical Immunology, Institut Curie, Paris, France. Here, at last, is a clear and concise guide to 100 of the most commonly used immunological techniques that can easily be performed by non-immunologists, and which assumes no prior knowledge of the techniques described. The idea for this book arose from the authors' observations that scientists in many fields of biomedical research needed, at some time or another, to perform an immunological technique applied to their own specific field of research. Existing manuals of immunological techniques are intended primarily for research immunologists and are either too detailed or assume background expertise that the user may not necessarily possess. Each technique is described step-by-step, in an easy-to-follow format, much like a cooking recipe, and is abundantly illustrated to give the user a clear understanding of what is happening at each stage. The book is edited by three experienced immunologists from the Curie Institute in Paris who have brought together an international panel of contributors, all of whom have hands-on expertise of the techniques they describe. Conveniently spiral-bound for easy use at the laboratory bench, the book will be a valuable resource for scientists who want a readily accessible reference to be able to perform immunological techniques successfully and painlessly. Zusammenfassung Mit Hilfe von Immunoassays bestimmt und quantifiziert man Antikörper. Laborhandbücher zu diesem Thema beschreiben meist hochspezialisierte Techniken, die eher Forscher als Routinelabors interessieren. Dieses Buch dagegen wendet sich insbesondere an Nichtfachleute: Schritt für Schritt werden leicht nachvollziehbare Methoden besprochen, zweifarbige Schemata erleichtern die Übersicht. Praktisch fürs Labor durch Spiralbindung. (05/98) Inhaltsverzeichnis CELLULAR IMMUNOLOGY TECHNIQUES. Cell Preparation. Cell Typing. Cytotoxicity. Cell Culture. Cell Activation. Cell Proliferation. Apoptosis Evaluation. IMMUNOCHEMICAL TECHNIQUES. Antigen Preparation. Generation of Polyclonal Antibodies. Generation of Monoclonal Antibodies and Hybridomas. Antibody Characterization. Antibody Purification. Biochemical Antibody Engineering. LABORATORY HOSPITAL TECHNIQUES. Appendices. Index....
Sommario
CELLULAR IMMUNOLOGY TECHNIQUES.
Cell Preparation.
Cell Typing.
Cytotoxicity.
Cell Culture.
Cell Activation.
Cell Proliferation.
Apoptosis Evaluation.
IMMUNOCHEMICAL TECHNIQUES.
Antigen Preparation.
Generation of Polyclonal Antibodies.
Generation of Monoclonal Antibodies and Hybridomas.
Antibody Characterization.
Antibody Purification.
Biochemical Antibody Engineering.
LABORATORY HOSPITAL TECHNIQUES.
Appendices.
Index.
