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This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Sommario
Methods for the Isolation and Recovery of Bifidobacteria.- Bifidobacterium Transformation.- Isolation of Chromosomal and Plasmid DNA from Bifidobacteria.- Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes.- Site-directed Mutagenesis of Bifidobacterium Strains.- Protocol to Select Bifidobacteria from Fecal and Environmental Samples.- Phageome Analysis of Bifidobacteria-Rich Samples.- Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria.- Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides.- Determination of Bifidobacterial Carbohydrate Utilisation Abilities and Associated Metabolic End-Products.- Model for Murine Gut Colonisation by Bifidobacteria.- Identification of Bifidobacteria by the Phosphoketolase Assay.- Determination of Bile Salt Hydrolase Activity in Bifidobacteria.- A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications.- Metagenomic Analyses of Bifidobacterial Communities.- Resistance of Bifidobacteria toward Antibiotics.- In vitro Assessment of Prebiotic Activity.- Bifidobacterium Genome Assembly and Methylome Analysis using Pacbio SMRT Sequencing.
