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This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models andmethods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them.
The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Sommario
Myocardial Infarction Techniques in Adult Mice.- Apical Resection and Cryoinjury in Neonatal Mouse Heart.- Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice.- Myocardial Infarction in Pigs.- Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish.- Cardiac Resection Injury in Zebrafish.- A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish.- Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration.- Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes.- Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes.- Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells.- In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells.- Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs.- Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells.- Isolation and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts.- Ex Vivo Techniques to Study Heart Regeneration in Zebrafish.- Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters.- In Vivo Clonal Analysis of Cardiomyocytes.- High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry.- AAV Gene Transfer to the Heart.- In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy.- Generation and Manipulation of Exosomes.- Epigenetic Assays in Purified Cardiomyocyte Nuclei.- Genetic Lineage Tracing of Non-Cardiomyocytes in Mice.- Experimental Hypoxia as a Model for Cardiac Regeneration in Mice.
