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Destined to set the standard, the first book dealing exclusively with this revolutionary and novel imaging technology serves as an easy-to- understand introduction while offering numerous tips and tricks.
Adopting a practical approach, the authors who developed this actual technique provide a comprehensive, hands-on overview of the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. As a reflection of the uncompromisingly interdisciplinary nature of the topic, they merge their expertise in physics, biology, and computer science, giving valuable insider tips taken from their work at major manufacturers.
The result is in-depth information on hardware and software solutions for a straightforward implementation of LSFM in the lab.
Sommario
LIGHT SHEET MICROSCOPY HARDWARE
Physics of Light Sheet-based Fluorescence Microscopes (e.g. Light Sheet formation, Optics, Detectors)
Overview of the Flavors of Light Sheet Microscopy (e.g. Arrangements of Optical Axes, Schematics, Commercial Set-ups, Extensions to Other Modalities)
Driving the Microscope and Open Access Electronics (e.g. Software to Pilot Light Sheet Microscopes (MicroManager), Arduino Solutions)
"Do It Yourself" Light Sheet based Microscope (e.g. OpenSPIM/OpenSPIN/LegoSPIM)
SAMPLE MOUNTING FOR LIGHT SHEET MICRSOCOPY
Sample Handling and Preparation (Including Sample Chamber Designs, Sample Positioning)
Clearing of Biological Tissues For Light Sheet Microscopy
Operation of Light Sheet-based Fluorescence Microscopes (Including In Multi-user Environment)
LIGHT SHEET MICRSOCOPY SOFTWARE
Image Processing of Multi-view Data (Registration, Fusion, and Deconvolution))
Visualization of Big Image Data (e.g. Local and Remote Viewing, 3D Rendering, Annotation)
Information Technology of Big Data Handling (e.g. Compression, Storage, Transfer)
Extracting Information From Light Sheet Datasets (e.g. Image Analysis Such As Atlas Registration, Segmentation, Tracking)
LIGHT SHEET MICROSCOPY APPLICATIONS
Applications In Developmental Biology
Applications In Cell Biology
Applications In Neurobiology
Applications In Biodiversity
LIGHT SHEET MICROSCOPY IMPLEMENTATIONS
Imaging Platform Integration
Laboratory Integration
IT Supports
Info autore
Emmanuel G. Reynaud, PhD, is Assistant Professor in Bioengineering in Biology at University College Dublin, Ireland. Pavel Toman¿ák, PhD, is Senior Permanent Research Group Leader at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany, and Director of the CEITEC Brno, Czechia.
Riassunto
Destined to set the standard, the first book dealing exclusively with this revolutionary and novel imaging technology serves as an easy-to- understand introduction while offering numerous tips and tricks.
Adopting a practical approach, the authors who developed this actual technique provide a comprehensive, hands-on overview of the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. As a reflection of the uncompromisingly interdisciplinary nature of the topic, they merge their expertise in physics, biology, and computer science, giving valuable insider tips taken from their work at major manufacturers.
The result is in-depth information on hardware and software solutions for a straightforward implementation of LSFM in the lab.
