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This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.
Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Sommario
Molecular Biology of Eosinophils: Introduction.- Functionally Active Eosinophil Purification from Peripheral Blood.- Eosinophil Purification from Peripheral Blood of Rhesus Monkeys.- Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors.- Detection of Mouse Eosinophils in Tissue by Flow Cytometry and Isolation by Fluorescence-Activated Cell Sorting (FACS).- Mutant Mice and Animal Models of Airway Allergic Disease.- Eosinophils in Asthma Models to House Dust Mite for Drug Development.- Analysis of Mouse Eosinophil Migration and Killing of Tumor Cells.- Murine Models of Eosinophil Function in Fungal and Viral Infections.- Assays of Eosinophil Apoptosis and Phagocytic Uptake.- In Vivo Eosinophil Expansion Using Gene Transfer by Electroporation.- Observation and Quantification of Eosinophil Motility.- Isolation and Functional Aspects of Eosinophil-Derived Exosomes.- Interactions of Eosinophils with Nerves.- Measurement of Eosinophil Kinetics In Vivo.- Detection of Eosinophil Extracellular DNA Traps.- Eosinophil Shape Change and Secretion.- Update on Eosinophils Interaction with Mast Cells: The Allergic Effector Unit.- Assessing Phenotypic Heterogeneity in Intestinal Tissue Eosinophils.- Antimicrobial Activity of Human Eosinophil Granule Proteins.- Experimental Modeling of Eosinophil-Associated Diseases.
