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This volume details protocols for studying Schistosoma mansoni. Chapters guide readers through reviews on current drugs and drug discovery, methods to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from S. mansoni, describes a drug screening, and how to test potential vaccine targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Schistosoma mansoni: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Sommario
Praziquantel - An Enigmatic, Yet Effective Drug.- Computer-aided Drug Design for the Identification of Novel Antischistosomal Compounds.- Immunofluorescent Localization of Proteins in Schistosoma mansoni.- Sensitive Fluorescence in situ hybridization on Semi-thin Sections of Adult Schistosoma mansoni using DIG-labeled RNA Probes.- Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni.- Labeling of the Schistosoma mansoni Tegument.- A Procedure for Analyzing the Proteomic Profile of Schistosoma mansoni Cercariae.- Proteomic Analysis of Schistosoma mansoni tegumental Proteins.- Measuring Histone Modifications in the Human Parasite Schistosoma mansoni.- Step-by-Step Bioinformatics Analysis of Schistosoma mansoni Long Non-coding RNA Sequences.- Identification of Transposable Elements in Schistosoma mansoni.- Structural and Functional Characterization of Schistosoma mansoni Cathepsin B1.- Characterisation of Schistosoma mansoni dihydrofolate Reductase (DHFR).- Characterisation of Schistosoma mansoni Glutamate-Gated Chloride Channels.- A Modified ELISA Method to Evaluate the Interaction of Schistosoma mansoni Proteins with Plasminogen.- Biophysical analysis of Schistosoma mansoni septins.- Crystallographic Studies of Triosephosphate Lsomerase from Schistosoma mansoni.- Luminescence-based, Low- and medium-throughput Assays for Drug Screening in Schistosoma mansoni Larval Stage.- Testing of Schistosoma mansoni Vaccine Targets.
