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This comprehensive book highlights the importance of Cyanidioschyzon merolae (C. merolae), an ultrasmall unicellular red alga, as a model eukaryote organism. The chapters introduce recent studies on C. merolae, from culture, synchronization and isolation methods of nucleic acids, proteins and organelles for molecular biological and cytological analyses, as well as its application in genetic engineering of environmental-stress-tolerant crops and oil production. In addition to discussing recent advances based on the complete genome information and molecular biological techniques such as genetic modifications and bioinformatics, the book includes visualization aids demonstrating that both classical and recent imaging techniques of fluorescent and electron microscopy can be applied to analyses of C. merolae. This publication offers a definitive resource for both beginners and professionals studying C. merolae, particularly in the field of molecular biology, evolutionary biology, morphology, biochemistry and cell biology, as well as those interested in its applications in medical sciences and agriculture.
Sommario
Preface.- Part I. Birth, evolution and structure of primary eukaryote.- Chapter 1. The Genus Cyanidiales: evolution and habitat.- Chapter 2. Cellular structure of Cyanidioschyzon merolae : minimum set of organelles.- Part II. Synchronous culture of Cyanidioschyzon merolae and isolation of organelles.- Chapter 3. Cultivation, observation and application of conventional experimental procedures.- Chapter 4. Isolation of (dividing) organelles.- Part III. Genome and post-genomic analyses of Cyanidioschyzon merolae.- Chapter 5. 100% complete nuclear and organellar genome sequences.- Chapter 6. "Omics" analyses of Cyanidioschyzon merolae based on complete genome information.- Chapter 7. Procedures for transformation and their application.- Part IV. Techniques for Morphological and Structural Biology .- Chapter 8. Basic techniques for fluorescence and electron microscopy.- Chapter 9. Cytological analyses by advanced electron mic
roscopy.- Chapter 10. Structural analyses by cryogenic coherent X-ray diffraction imagingPart V. Proliferation of Eukaryotic Cell.- Chapter 11. Cell cycle and organelle division cycle.- Chapter 12. Synchronization of cell cycle by light and circadian rhythms.- Chapter13. Control of cell nuclear DNA replication by chloroplast and mitochondrion.- Chapter 14. Double-membrane-bounded organelles.- Chapter 15. Single-membrane-bounded organelles.- Chapter 16. Control of organelle division.- Part VI. Metabolism.- Chapter 17. Photosynthesis.- Chapter 18. Nitrogen metabolism.- Chapter 19. Carbon metabolism.- Part VII. Application.- Chapter 20. Application of the tolerance to extreme environment to other organisms.- Chapter 21. Metabolic engineering.- Appendix.- Index.
Info autore
Tsuneyoshi Kuroiwa Biological Imaging Center, Japan Women's University
Tokyo, Japan
Shinya Miyagishima Department of Cell Genetics, National Institute of Genetics
Shizuoka, Japan
Sachihiro Matsunaga Dept of Applied Biological Science, Tokyo University of Science
Chiba, Japan
Naoki Sato Department of Life Sciences, University of Tokyo
Tokyo, Japan
Hisayoshi Nozaki Department of Biological Science, University of Tokyo
Tokyo, Japan
Kan Tanaka Chemical Resources Laboratory, Tokyo Institute of Technology
Kanagawa, Japan
Osami Misumi
Dept of Biological Science and Chemistry, Yamaguchi University
Yamaguchi, Japan
Riassunto
This comprehensive book highlights the importance of Cyanidioschyzon merolae (C. merolae), an ultrasmall unicellular red alga, as a model eukaryote organism. The chapters introduce recent studies on C. merolae, from culture, synchronization and isolation methods of nucleic acids, proteins and organelles for molecular biological and cytological analyses, as well as its application in genetic engineering of environmental-stress-tolerant crops and oil production. In addition to discussing recent advances based on the complete genome information and molecular biological techniques such as genetic modifications and bioinformatics, the book includes visualization aids demonstrating that both classical and recent imaging techniques of fluorescent and electron microscopy can be applied to analyses of C. merolae. This publication offers a definitive resource for both beginners and professionals studying C. merolae, particularly in the field of molecular biology, evolutionary biology, morphology, biochemistry and cell biology, as well as those interested in its applications in medical sciences and agriculture.
