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This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.
The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.
Sommario
Culture of Human Monocyte-Derived Macrophages.- A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages.- Growing Murine Bone Marrow-Derived Macrophages.- Immortalization of Murine Bone Marrow-Derived Macrophages.- Isolation and Identification of Murine Serous Cavity Macrophages.- Isolation and Identification of Interstitial Macrophages from the Lungs using Different Digestion Enzymes and Staining Strategies.- Isolation and Phenotyping of Adult Mouse Microglial Cells.- Isolation and Phenotyping of Bone Marrow Macrophages.- Isolation and Phenotyping of Intestinal Macrophages.- Phenoytyping Tumor Associated Macrophages.- Activating Murine Macrophages In Vitro.- Polarizing Macrophages In Vitro.- Viral Replication Assay in Bone Marrow-Derived Macrophages.- Macrophage Bactericidal Assays.- Quantitative Phagocytosis Assays in Primary and Cultured Macrophages.- Observing Frustrated Phagocytosis and Phagosome Formation and Closure using Total Internal Reflection Fluorescence Microscopy (TIRFM).- Chromatine Immunoprecipitation in Macrophages.- Transfecting Macrophages.- In Vitro Migration Assays.- Cytokine-Induced Acute Inflammatory Monarticular Arthritis.- Characterizing Activation, Proliferation, and Ontogeny of Murine Macrophages in Parasitic Helminth Infections.- Genetic Models of Macrophage Depletion.- Depleting Macrophages In Vivo with Clodronate-Liposomes.- Cre Driver Mice Targeting Macrophages.
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Riassunto
This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.
The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.
