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This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field.
Sommario
NMDA Receptors in the Central Nervous System.- Quantification of NMDAR Subunit Genes Expression by qRT-PCR.- Genetic and Functional Analysis of GRIN2A in Tumor Samples.- Detection of NMDARs Antibodies in Encephalitis.- MagnetofectionTM of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host Cells.- Transfection in Primary Cultured Neuronal Cells.- Selective Cell-Surface Expression of Triheteromeric NMDA Receptors.- Functional Analysis of Recombinant Channels in Host Cells using a Fast Agonist Application System.- GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging.- Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers.- Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations.- Electrophysiological Investigation of NMDA Current Properties in Brain Slices.- Analysis of Functional NMDA Receptors in Astrocytes.- GluNs Detection and Functions in Microglial Cells.- NMDA ReceptorActivity in Circulating Red Blood Cells: Methods of Detection.- NMDA Receptors as Voltage Sensors.- Development of a Computational Approach/Model to Explore NMDA Receptors Functions.
Riassunto
This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing.
