Ulteriori informazioni
This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Sommario
The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions.- Protocols of IATC, DSC, and PPC: The Multi-State Structural Transition of Cytochrome c.- Value of DSC in Characterization and Optimization of Protein Stability.- Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC).- Intrinsic Thermodynamics of Protein - Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design.- Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions.- ITC Studies of Ribosome/Antibiotics Interactions.- High Quality Data of Protein/Peptide Interaction by Isothermal Titration Calorimetry.- ITC Measurement for High Affinity Aptamers Binding to their Target Proteins.- Thermodynamics of Molecular Machines using Incremental ITC.- Measuring the Metabolic Activity of Mature Mycobacteria Biofilms using Isothermal Microcalorimetry.- Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC).- Analysis of Isothermal Titration Calorimetry Data for Complex Interactions using I2CITC.- Tinkering with Binding Polynomials in Isothermal Titration Calorimetry.- Use of ITC and the Software AFFINImeter for the Quantification of Anticoagulant Pentasaccharide in Low Molecular Weight Heparine.- Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by using kinITC in AFFINImeter.- Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry.- Characterization of Enzymatic Reactions using ITC.
Riassunto
This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.
