Vertebrate Embryogenesis - Embryological, Cellular, and Genetic Methods

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.  
Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Sommario

Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments.- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos.- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos.- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts.- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis.- Embryological and Genetic Manipulation of Chick Development.- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos.- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens.- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq).- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.- Ex Utero Culture and Imaging of Mouse Embryos.- Detection of Gene and Protein Expression in MouseEmbryos and Tissue Sections.- Reptile Embryology and Regeneration.- Manipulation of Developmental Function in Turtles with Notes on Alligators.- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes.- Visualizing the Balbiani Body in Zebrafish Oocytes.- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis.- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos.- Germ Cell Transplantation in Avian Species.- Production of Germ-Line Chimeras in Zebrafish.- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring.- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish.- Modeling Embryonic Cleavage Patterns.- Laboratory Guidelines for Animal Care.

Riassunto

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.  
Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Dettagli sul prodotto

• Con la collaborazione di: Francisc J Pelegri (Editore), Francisco J Pelegri (Editore), Francisco J. Pelegri (Editore)
• Editore: Springer, Berlin
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 31.01.2019
• EAN: 9781493990085
• ISBN: 978-1-4939-9008-5
• Pagine: 434
• Dimensioni: 193 mm x 64 mm x 258 mm
• Peso: 1010 g
• Illustrazioni: XI, 434 p. 82 illus., 70 illus. in color.
• Serie: Methods in Molecular Biology; Methods in Molecular Biology
• Categoria: Scienze naturali, medicina, informatica, tecnica > Biologia > Genetica, tecnica genetica