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This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities. Chapters in Biosensors and Biodetection: Methods and Protocols, Volume 1: Optical-Based Detectors, Second Edition chapters focus on direct and indirect optical detectors including surface plasmon resonance, interferometric sensors, charge-coupled device (CCD) based detectors, spectrometry, and many other cutting-edge optical biosensors technologies. Written in the format of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, tips on troubleshooting and avoiding known pitfalls, and step-by-step, readily reproducible laboratory protocols.
Authoritative and practical, Biosensors and Biodetection: Methods and Protocols, Volume 1: Optical-Based Detectors, Second Edition offers descriptions of major technologies by leading experts in the field in extensive technical detail. The aim of the book is to make biosensors more accessible and understandable to engineers, students, medical professionals, molecular biologists, chemical, and physical science researchers developing biosensor technologies, allowing readers to both understand the technology and to construct similar devices.
Sommario
Localized Surface Plasmon Resonance (LSPR)-Coupled Fiber-Optic Nanoprobe for the Detection of Protein Biomarkers.- Ultra-Sensitive Surface Plasmon Resonance Detection by Colocalized 3D Plasmonic Nanogap Array.- Two-dimensional Surface Plasmon Resonance Imaging (2D-SPR) System for Cellular Analysis.- Immunosensing with Near-infrared Plasmonic Optical Fiber Gratings.- Biosensing Based on Magneto-Optical Surface Plasmon Resonance.- Nanoplasmonic Biosensor Using Localized Surface Plasmon Resonance Spectroscopy for Biochemical Detection.- Plasmonics Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles.- MicroRNA Biosensing with Two-dimensional Surface Plasmon Resonance Imaging.- Gold Nanorod Array Biochip for Label-free, Multiplexed Biological Detection.- Resonant Waveguide Grating Imager for Single Cell Monitoring of the Invasion of Spheroidal Cancer Cells through Matrigel.- Label-Free Biosensors Based on BimodalWaveguide (BiMW) Interferometers.- DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection 'DNA-directed Antibody Immobilization'.- Reflectometric Interference Spectroscopy.- Hypermulticolor Detector for Quantum-antibody Based Concurrent Detection of Intracellular Markers for HIV Diagnosis.- Low Cost Charged-coupled Device (CCD) Based Detectors for Shiga Toxins Activity Analysis.- Smartphone-enabled Detection Strategies for Portable PCR-based Diagnostics.- Streak Imaging Flow Cytometer for Rare Cell Analysis.- Rapid Detection of Microbial Contamination Using a Microfluidic Device.- Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors.- Enhanced Performance of Colorimetric Biosensing on Paper Microfluidic Platforms through Chemical Modification and Incorporation of Nanoparticles.- A Smartphone-based Colorimetric Reader for Human C-reactive Protein Immunoassay.- A Novel Colorimetric PCR-based Biosensor for Detection and Quantification of Hepatitis B Virus.- CCD Camera Detection of HIV Infection.- "Dipstick" Colorimetric Detection of Metal ions based on Immobilization of DNAzyme and Gold Nanoparticles onto a Lateral Flow Device.- Liposome-enhanced Lateral-flow Assays for Clinical Analyses.- Development of Dual Quantitative Lateral Flow Immunoassay for the Detection of Mycotoxins.
Riassunto
This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities. Chapters in Biosensors and Biodetection: Methods and Protocols, Volume 1: Optical-Based Detectors, Second Edition chapters focus on direct and indirect optical detectors including surface plasmon resonance, interferometric sensors, charge-coupled device (CCD) based detectors, spectrometry, and many other cutting-edge optical biosensors technologies. Written in the format of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, tips on troubleshooting and avoiding known pitfalls, and step-by-step, readily reproducible laboratory protocols.
Authoritative and practical, Biosensors and Biodetection: Methods and Protocols, Volume 1: Optical-Based Detectors, Second Edition offers descriptions of major technologies by leading experts in the field in extensive technical detail. The aim of the book is to make biosensors more accessible and understandable to engineers, students, medical professionals, molecular biologists, chemical, and physical science researchers developing biosensor technologies, allowing readers to both understand the technology and to construct similar devices.
