Ulteriori informazioni
This book provides an overview of single-cellisolation, separation, injection,lysis and dynamics analysis as well as a study of their heterogeneity usingdifferent miniaturized devices. As an important part of single-cell analysis, differenttechniques including electroporation,microinjection, optical trapping, optoporation, rapid electrokinetic patterningand optoelectronictweezers are described in detail. It presents different fluidicsystems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry)and their integration with sensor technology, optical and hydrodynamic stretchers etc.,and demonstrates the applications of single-cell analysis in systems biology,proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics,biomedicine and drug delivery systems. It also discusses the future challengesfor single-cell analysis, including the advantages and limitations.
This book is enjoyable reading material whileat the same time providing essential information to scientists in academia andprofessionals in industry working on different aspects of single-cellanalysis.
Dr. Fan-Gang Tseng is a Distinguished Professor ofEngineering and System Science atthe National Tsing Hua University, Taiwan.
Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Sommario
SingleCell Behavioral Assays for Heterogeneity Studies.- Systems biology in single cells.- Electroporation for single cellanalysis.- Microinjection for single cellanalysis.- Optical tools for single cellmanipulation and analysis.- Optoelectrokinetic manipulationfor cell analysis.- Continuous micro/nano-fluidicdevices for single cell analysis.- Single Cell MechanicalProperties: Label-Free Biomarkers for Cell Status Evaluation.- Cytometry of single cells forbiology and biomedicine.- Single cell genomics andepigenomics.- Single cell Metabolomics.- Applications of cell based drugdelivery systems: use of single cell assay.- Applications of Single CellSequencing in Cancer.- Single Cell Characterization ofMicroalgal Lipid Contents with Confocal Raman Microscopy.- Single Differentiated Neurons fromPluripotent Embryonic Stem Cells: Motor Protein Modelling and NeurodegenerativeDisease.
Info autore
Dr.
Fan-Gang Tseng received his Ph.D. degree in mechanical engineering from UCLA,
USA, in 1998. He is currently a Distinguished
Professor
in the ESS Department
as well as at the NEMS Institute, and the
Deputy Director of the Biomedical Technology Research Center at NTHU. He is
also an affiliated Research Fellow of Academia Sinica, Taiwan. He was elected as
an ASME fellow in 2014. His research interests are in the fields of BioNEMS,
Biosensors, Micro-Fluidics, Tissue Chips, and Fuel Cells. He holds 60 patents, authored 8 book chapters, and
published more than
220 journal papers and 360 conference papers. Dr. Tseng currently serves on the
editorial boards of Applied Sciences, Journal of Circuits and Systems,
International Journal of Molecule Science, Micromachines, and Open Nanomedicine
Journal. He received many awards, including National
Innovation Award (twice), Outstanding Research Award (MOST and NSC, twice), First-Class Research
Award (NSC), Mr. Wu, Da-Yo Memorial Award (NSC), NTHU Outstanding Research Award (2005-2015), and more than twelve best
paper/poster awards from various competitions and conferences.
Dr. Tuhin Subhra Santra
received
his Ph.D. degree in Bio-Nano Electro Mechanical Systems (Bio-NEMS) from the
Institute of Nanoengineering and Microsystems (NEMS), National Tsing Hua
University (NTHU), Taiwan in 2013. Currently he is a Postdoctoral Research Associate at the California Nano System Institute (CNSI),
University of California, Los Angeles (UCLA), USA. His main research areas are
Bio-NEMS, MEMS, Single-Cell Analysis, Bio-micro/nano
Fabrication, Biomedical Microdevices, Nanomedicine and etc. Dr. Santra serves
as a Guest Editor for the Journal of Micromachines, Journal of Molecules,
International Journal of Molecular Science, Sensors and Transducers Journal,
American Journal of Nanoresearch and Application, among others. Dr. Santra has
received many awards such as the NTHU Outstanding Student Award in 2011 and
2013, IEEE-NEMSBest Conference Paper Award in 2014, Best Poster Award at 15th
Nano/Microsystem Conference in 2012, Junior Research
Fellowship from the
IIT-Kharagpur in 2008, Outstanding
Student Award from the Jadavpur University in
2005 and 2006, Silver Medal from the Vidyasagar
University in 2004. Dr. Santra has published more than 15 international journal
papers, six book chapters, 31 international conference papers in his research
field. He also has one US and one Taiwan patent
pending.
Riassunto
This book provides an overview of single-cell
isolation, separation, injection,
lysis and dynamics analysis as well as a study of their heterogeneity using
different miniaturized devices. As an important part of single-cell analysis, different
techniques including electroporation,
microinjection, optical trapping, optoporation, rapid electrokinetic patterning
and optoelectronic
tweezers are described in detail. It presents different fluidic
systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry)
and their integration with sensor technology, optical and hydrodynamic stretchers etc.,
and demonstrates the applications of single-cell analysis in systems biology,
proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics,
biomedicine and drug delivery systems. It also discusses the future challenges
for single-cell analysis, including the advantages and limitations.
This book is enjoyable reading material while
at the same time providing essential information to scientists in academia and
professionals in industry working on different aspects of single-cell
analysis.
Dr. Fan-Gang Tseng is a Distinguished Professor of
Engineering and System Science at
the National Tsing Hua University, Taiwan.
Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
