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Transposable elements are used as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change and diversity, and the evolutionary impact of mobile DNA on the biology and evolution of organism. In, Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition gathers a wide array of strategies and protocols for identifying transposable elements (TEs) and their evolutionary derivatives, and for studying genome structure, function, and evolution. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Through and intuitive Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition aids scientists in the continuing study of in silico tools, strategies and protocols that are complementary of those described in the first volume.
Sommario
Transposable Elements as Tools for Reshaping the Genome: it's a Huge World After All !.-Using and Understanding RepeatMasker.-Roadmap for Annotating Transposable Elements in Eukaryote Genomes.-To Detect and to Analyze Sequence Repeats Whatever their Origin.-Exploring Bacterial Insertion Sequences with ISfinder: Objectives, Uses and Future Developments.-Methods and Software in NGS for TE Analysis.-The Application of LTR Retrotransposons As Molecular Markers in Plants.-Individual Analysis of Transposon Polymorphisms by AFLP.-Construction of a Library of Random Mutants in the Spirochete Leptospira Biflexa using a Mariner Transposon.-Ac-Ds Solutions for Rice Insertion Mutagenesis.-Engineering the Caenorhabditis elegans Genome by Mos1 Induced Transgene-Instructed Gene Conversion (MosTIC).-Genome-wide Manipulations of Drosophila Melanogaster with Transposons, Flp Recombinase and FC31 Integrase.-The Sleeping Beauty Transposon Toolbox.-PiggyBac tool box.-Insertion Site Pattern: Global Approach by Linear Amplification-mediated PCR (LAM-PCR) and Mass Sequencing.-Comprehensive DNA Methylation Profiling of Human Repetitive DNA Elements using a MeDIP-on-RepArray Assay.-Novel Approach for the Development of New Antibodies Directed Against Transposase-derived Proteins Encoded by Human Neogenes.
Riassunto
Transposable elements are used as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change and diversity, and the evolutionary impact of mobile DNA on the biology and evolution of organism.
