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Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs. In Vaccine Adjuvants: Methods and Protocols, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products. Rather than describing individual adjuvants, the volume strives to include detailed, practical information on measuring the responses produced by adjuvants in order to be relevant to the widest array of experiments. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and versatile, Vaccine Adjuvants: Methods and Protocols will enable those already pursuing vaccine adjuvant research, while also serving to stimulate discussion on how to best standardize adjuvant testing in order to facilitate meaningful comparisons, and above all, to aid in the prediction of which new products will most effectively and safely help to solve the current challenges in vaccination.
Sommario
Immunology of Vaccine Adjuvants.- Preclinical Development of AS04.- Nonclinical Safety Assessment of Vaccines and Adjuvants.- Aluminum Adjuvants: Preparation, Application, Dosage, and Formulation with Antigen.- Freund's Complete and Incomplete Adjuvants, Preparation, and Quality Control Standards for Experimental Laboratory Animals Use.- Liposomal Adjuvants: Preparation and Formulation with Antigens.- Micro/Nanoparticle Adjuvants: Preparation and Formulation with Antigens.- Adjuvant Activity on Human Cells In Vitro.- Adjuvant Activity on Murine and Human Macrophages.- In Vitro Effects of Adjuvants on B Cells.- NKT Cell Responses to Glycolipid Activation.- Tracking Dendritic Cells In Vivo.- Adjuvant Effects on Antibody Titre.- Functional Antibody Assays.- Determining Adjuvant Activity on T-Cell Function In Vivo: Th Cells.- Quantitative Multiparameter Assays to Measure the Effect of Adjuvants on Human Antigen-Specific CD8 T-Cell Responses.- Large-Animal Model for Establishing E/T Ratio of Adjuvants.- Determining the Activity of Mucosal Adjuvants.- Adjuvant Activity of Cytokines.
Riassunto
Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs.
