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The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.
Sommario
Breast Cancer Stem Cells: Current Advances and Clinical Implications.- A Protocol for Studying Embryonic Mammary Progenitor Cells During Mouse Mammary Primordial Development in Explant Culture.- FACS Sorting Mammary Stem Cells.- Side Population.- Single Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing.- Shotgun Proteomics on Tissue Specimens Extracted with Acid Guanidinium-Thiocyanate-Phenol-Chloroform.- Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments.- Lentiviral Transduction of Mammary Epithelial Cells.- The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads.- Humanization of the Mouse Mammary Gland.- Lineage Tracing in the Mammary Gland Using Cre/lox Technology and Fluorescent Reporter Alleles.- Modeling the Breast Cancer Bone Metastatic Niche in Complex Three Dimensional Co-Cultures.- Mammary Cancer Stem Cells Re-Initiation Assessment at the Metastatic Niche: The Lung and Bone.- Nanomechanical Characterization of Living Mammary Tissues by Atomic Force Microscopy.- Mathematical Modeling as a Tool to Understand Cell Self-Renewal and Differentiation.- Mammary Stem Cells: A Clinician´s View.
Info autore
María dM Vivanco, PhD CIC bioGUNE Cell Biology & Stem Cells Unit Parque Tecnológico de Bizkaia, Ed 801A 48160 Derio (Bizkaia) Spain Tel: +34 94 406 1322 email: mdmvivanco@cicbiogune.es
Riassunto
The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.
