Ulteriori informazioni
T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.
Sommario
Isolation of T Cells from the Skin.- Isolation of Leukocytes from Mouse Central Nervous System.- Isolation of T Cells from the Gut.- T Cell Isolation from Mouse Kidneys.- Intracellular Staining for Cytokines and Transcription Factors.- Surface Capture Staining.- Mass Cytometry Analysis of Human T Cell Phenotype and Function.- Detection of NF- B Pathway Activation in T Helper Cells.- Assessing the Suppressive Activity of Foxp3+ Regulatory T Cells.- In vitro Generation of Microbe Specific Human Th17 Cells.- In vitro Polarization of T-Helper Cells.- A Method for the Generation of TCR Retrogenic Mice.- Mouse Models of Allergic Airway Disease.- Induction of Colitis in Mice (T-Cell Transfer Model).- Manipulation of T Cell Function Conditional Gene Targeting in T Cells.- Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes.- Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells.- Experimental Mouse Models of T Cell-Dependent Inflammatory Bowel Disease.- Analysis of Chromosomal Aberrations in Murine HCC.
Riassunto
T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.
Testo aggiuntivo
From the book reviews:
“This is a useful source of current protocols designed to capture the unique features of T helper cells with tools developed for the isolation of these cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo, and in vivo. … This book is intended for all interested in learning about protocols and methods in T helper cells. Professional laboratory scientists as well as beginners in the field of immunology will find this book extremely useful.” (Omer Iqbal, Doody’s Book Reviews, January, 2015)
Relazione
From the book reviews:
"This is a useful source of current protocols designed to capture the unique features of T helper cells with tools developed for the isolation of these cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo, and in vivo. ... This book is intended for all interested in learning about protocols and methods in T helper cells. Professional laboratory scientists as well as beginners in the field of immunology will find this book extremely useful." (Omer Iqbal, Doody's Book Reviews, January, 2015)
