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Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.
Sommario
Toxicological Aspects for Nanomaterial in Humans.- Lactate Dehydrogenase Assasy for Assessment of Polycation Cytotoxicity.- Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determincation for Assessment of Polycation-Mediated Cytotoxicity.- Anti-PEG IgM Production Via a PEGylated Nano-Carrier System for Nucleic Acid Delivery.- Near-Infrared Optical Imaging of Nucleic Acid Nanocarrier In Vivo.- Lipids for Nucleic Acid Delivery: Synthesis and Particle Formantion.- Histidine-Rich Cationic Amphipathic Peptides for Plasmid DNA and siRNA Delivery.- Synthesis of Polyethylenimine Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids.- Synthesis of Bioreducible Polycations with Controlled Topologies.- Lyophilization of Synthetic Gene Carriers.- Surface-and Hydrogel-Mediated Delivert of Nucleic Acid Nanoparticles.- Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids.- In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films.- Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles.- Magnetic And Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery.- Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery.- Lipopeptide Delivery of siRNA to the Central Nervous System.- Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery.- Liver-Targeted Gene Delivery Through Retrograde IntrabiliaryInfusion.
Riassunto
Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way.
