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In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.
Sommario
Culturing Larvae of Marine Invertebrates.- Obtaining Gametes and Embryos of Ascidians.- Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis.- Trichoplax adhaerens, an Enigmatic Basal Metazoan with Potential.- Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses.- An Invertebrate Embryologist's Guide to Routine Processing of Confocal Images.- TEM Analyses of Chaetognath Reproductive Organs.- SEM Analysis of Marine Invertebrate Gametes.- Imaging Neural Development in Embryonic and Larval Sea Urchins.- Preparation and Use of Sea Urchin Egg Homogenates.- Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata.- Isolating Specific Embryonic Cells of the Sea Urchin by FACS.- Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata.- Perturbations to the Hedgehog Pathway in Sea Urchin Embryos.- Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin in vivo.- Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte .- Maturation in Marine Nemertean Worms.- Multi-color Labeling in Developmental Gene Regulatory Network Analysis.- A 96-well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay.- Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchines.- Tools for Sea Urchin Genomic Analysis.- Antibody Inhibition of Protein Activity in Starfish Oocytes.- Immunophotoaffinity Labeling of the Binding Proteins for 1-Methyladenine, an Oocyte-maturation Inducing Hormone of Starfish.
Riassunto
In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.
