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This book contemplates the structure, dynamics and physics of virus particles: From the moment they come into existence by self-assembly from viral components produced in the infected cell, through their extracellular stage, until they recognise and infect a new host cell and cease to exist by losing their physical integrity to start a new infectious cycle. (Bio)physical techniques used to study the structure of virus particles and components, and some applications of structure-based studies of viruses are also contemplated. This book is aimed first at M.Sc. students, Ph.D. students and postdoctoral researchers with a university degree in biology, chemistry, physics or related scientific disciplines who share an interest or are actually working on viruses. We have aimed also at providing an updated account of many important concepts, techniques, studies and applications in structural and physical virology for established scientists working on viruses, irrespective of their physical, chemical or biological background and their field of expertise. We have not attempted to provide a collection of for-experts-only reviews focused mainly on the latest research in specific topics; we have not generally assumed that the reader knows all of the jargon and all but the most recent and advanced results in each topic dealt with in this book. In short, we have attempted to write a book basic enough to be useful to M.Sc and Ph.D. students, as well as advanced and current enough to be useful to senior scientists with an interest in Structural and/or Physical Virology.
Sommario
Introduction: the structural basis of virus function.- The basic architecture of viruses.- Conventional electron microscopy, cryo-electron microscopy and cryo-electron tomography of viruses.- X-ray crystallography of viruses.- Nuclear magnetic resonance spectroscopy to study virus structure.- Fluorescence, circular dichroism and mass spectrometry as tools to study virus structure.- Combined approaches to study virus structure.- Atomic force microscopy of viruses.- Optical tweezers to study viruses.- Assembly of simple icosahedral viruses.- Structure and assembly of complex viruses.- Nucleic acid packaging in viruses.- Virus maturation.- Virus morphogenesis in the cell: methods and observations.- Virus-receptor interactions and receptor-mediated virus entry into host cells.- Entry of enveloped viruses into host cells: membrane fusion.- Bacteriophage receptor recognition and nucleic acid transfer.- Mechanical properties of viruses.- Theoretical studies on assembly, physical stabilityand dynamics of viruses.- Antiviral agents: Structural basis of action and rational design.- Design of novel vaccines based on virus-like particles or chimeric virions.- Nanoscale science and technology with plant viruses and bacteriophages.
Riassunto
This book contemplates the structure, dynamics and physics of virus particles: From the moment they come into existence by self-assembly from viral components produced in the infected cell, through their extracellular stage, until they recognise and infect a new host cell and cease to exist by losing their physical integrity to start a new infectious cycle. (Bio)physical techniques used to study the structure of virus particles and components, and some applications of structure-based studies of viruses are also contemplated. This book is aimed first at M.Sc. students, Ph.D. students and postdoctoral researchers with a university degree in biology, chemistry, physics or related scientific disciplines who share an interest or are actually working on viruses. We have aimed also at providing an updated account of many important concepts, techniques, studies and applications in structural and physical virology for established scientists working on viruses, irrespective of their physical, chemical or biological background and their field of expertise. We have not attempted to provide a collection of for-experts-only reviews focused mainly on the latest research in specific topics; we have not generally assumed that the reader knows all of the jargon and all but the most recent and advanced results in each topic dealt with in this book. In short, we have attempted to write a book basic enough to be useful to M.Sc and Ph.D. students, as well as advanced and current enough to be useful to senior scientists with an interest in Structural and/or Physical Virology.
