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In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances, drug discovery and delivery modalities, animal and humanized models and new applications in the treatment of pathological wounds in a variety of organs and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, a detailed list of the necessary materials and reagents for each procedure, step-by-step, reproducible laboratory protocols, and a set notes, developed by the authors, for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Wound Regeneration and Repair: Methods and Protocols seeks to aid scientists and entrepreneurs in their further study of technologies, models, techniques, and critical new areas and approaches to clinical and commercial translation of research.
Sommario
An In Vivo Model System for Evaluation of the Host Response to Biomaterials.- Urothelial Cell Culture.- Cell Populated Collagen Lattices Contraction Model for the Investigation of Fibroblast Collagen Interactions.- A Tissue-engineered Corneal Wound Healing Model for the Characterization of Reepithelialization.- Adult Stem Cells in Small Animal Wound Healing Models.- Novel Animal Models for Tracking the Fate and Contributions of Bone Marrow Derived Cells in Diabetic Healing.- Cell-Based Therapies for Myocardial Repair: Emerging Role for Bone Marrow Derived Mesencymal Stem Cells (MSCs) in the Treatment of the Chronically Injured Heart.- A Model System for Primary Abdominal Closures.- Alternatives for Animal Wound Model Systems.- Novel Methods for the Investigation of Human Hypertrophic Scarring and other Dermal Fibrosis.- Study of the Human Chronic Wound Tissue: Addressing Logistic Barriers and Productive use of Laser Capture Microdissection.- The Wound Watch: An Objective Staging System for Wounds in the Diabetic (db/db) Mouse Model.- Human Ex vivo Wound Healing Model.- Murine Models of Human Wound Healing.- A Corneal Scarring Model.- A Novel and Efficient Model of Coronary Artery Ligation in the Mouse.- Cardiac Wound Healing Post-Myocardial Infarction: A Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle.- Injury Models to Study Cardiac Remodeling in the Mouse: Myocardial Infarction and Ischemia-reperfusion.- Cryoinjury Models of the Adult and Neonatal Mouse Heart for Studies of Scarring and Regeneration.- Targeting Wnt Signaling to Improve Wound Healing after Myocardial Infarction.- Vascular Connexins in Restenosis after Balloon Injury.- Gain-of-function Assays in the Axolotl (Ambystoma mexicanum) to Identify SignalingPathways that Induce and Regulate Limb Regeneration.- The Mouse Digit Tip: from Wound Healing to Regeneration.- Lower Vibrissa Follicle Amputation: A Mammalian Model of Regeneration.- Using Drosophila Larvae to Study Epidermal Wound Closure and Inflammation.- Zebrafish Cardiac Injury and Regeneration Models: A Non-invasive and Invasive in vivo Model of Cardiac Regeneration.- Quantifying Alterations in Cell Migration: Tracking Fluorescently Tagged Migrating Cells by FACs and Live Imaging.- Examining the Role of Mast Cells in Fetal Wound Healing using Cultured Cells in vitro.- Assessing Macrophage Phenotype During Tissue Repair.- The use of Connexin-Based Therapeutic Approaches to Target Inflammatory Diseases.- Commercialization: Patenting and Licensing in Wound Healing and Regenerative Biology.- Translational Strategies for the Development of a Wound Healing Technology (idea) from Bench to Bedside.
Riassunto
In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances, drug discovery and delivery modalities, animal and humanized models and new applications in the treatment of pathological wounds in a variety of organs and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, a detailed list of the necessary materials and reagents for each procedure, step-by-step, reproducible laboratory protocols, and a set notes, developed by the authors, for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Wound Regeneration and Repair: Methods and Protocols seeks to aid scientists and entrepreneurs in their further study of technologies, models, techniques, and critical new areas and approaches to clinical and commercial translation of research.
