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This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.
Sommario
Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons.- Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy.- Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei.- Nuclear Signs of Pre-Neurodegeneration.- Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes.- Calcium Imaging in Neuron Cell Death.- Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis.- Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET).- Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to .- Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina.- Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis.- High-Throughput Cell Death Assays.- Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death.- In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death.- Laser Microbeam Targeting of Single Nerve Axons in Cell Culture.- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy.- Targeted Toxicants to Dopaminergic Neuronal Cell Death.- Stem Cells, Neural Progenitors, and Engineered Stem Cells.- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene Therapy.- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene Therapy.- Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous System.- Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA).- Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing.- InVivo Assessment of Neuronal Cell Death in Drosophila.- Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death.
Riassunto
This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.
