Ulteriori informazioni
Das Praxisbuch zur DC jetzt auch in englischer Sprache!Die Dünnschicht-Chromatographie ist eine schnelle und preiswerte Analysemethode mit Anwendungen, die sich u. a. in der Arzneimittel-, Lebensmittel- und Umweltanalytik bewährt hat. Das Buch ist ein Leitfaden für die praktische Arbeit und eine wertvolle Hilfe bei der Fehlersuche und -vermeidung. Das Praxisbuch zur DC jetzt auch in englischer Sprache!Aus einer Besprechung der deutschen Ausgabe:'Die Summe von praktischen Tips macht das Buch zu einem Nachschlagewerk, das in jedes Labor gehört, wo dünnschichtchromatographisch gearbeitet wird. ... Durch die konsequente Erläuterung aller, auch für den Praktiker geläufigen, Vorgehensweisen und Begriffe und seiner sehr einfachen Sprache eignet sich das Buch sehr gut als Lehrbuch und wird sicherlich bei der Ausbildung von Laboranten in der Pharmazeutischen und Chemischen Industrie sowie von Apothekern und PTA's eine weite Verbreitung finden.'(Deutsche Apotheker Zeitung)
Sommario
INTRODUCTIONWhat is TLC?When is TLC used?Where is TLC usedHow is the result of a TLC represented?What kinds of reference substances are used in TLC?The literature on TLCPRECOATED LAYERSWhy use precoated layers?How are precoated layers produced?What kinds of precoated layers are there?What are precoated layers used for?Criteria for choosing stationary phases in TLCEffect of the stationary phase when the solvents are identicalRecommendations for the procurement and storage of precoated layersProblems in the nomenclature and classification of precoated layersBEFORE TLC DEVELOPMENTHandling of the precoated layersPrewashingActivationConditioningImpregnationApplication of the samplePositioning of the sampleDrying before developmentSOLVENT, DEVELOPING CHAMBERS AND DEVELOPMENTSolventTLC developing chambersDevelopment of thin-layer chromatogramsDrying after developmentEVALUATION WITHOUT DERIVATIZATIONVisual direct evaluationOptical direct evaluation using special equipmentCoupling methods for substance identificationDocumentation without or before derivatizationDERIVATIZATIONThermochemical reactionIrradiation with high energy lightReaction with reagentsSpecial types of derivatizationTreatment of derivatized chromatogramsEVALUATION AFTER DERIVATIZATIONVisual evaluationEvaluation by TLC scannerEvaluation by video systemEvaluation by flat-bed scannerDOCUMENTATIONDescription of a thin-layer chromatogramDocumentation by reproduction, tracing or photocopyingPhotographic documentationVideo documentationTLC scanner documentationFlat-bed scanner documentationGMP/GLP-CONFORMING TLC WORKValidation of TLC methodsUse of qualifiedcalibrated equipmentGMP/GLP-conforming rawdata sheetsExamples of GMP/GLP-conforming testing proceduresLOADING EFFECTSDefinite loading of a substanceTLC of sensitive substancesSPECIAL METHODS IN TLCAMD - Automated Multiple DevelopmentOPLC - Overpressured Layer ChromatographyHPPLC - High Pressure Planar Liquid ChromatographyTLC-FID/FTID - Combination of TLC with Flame Ionization Detector or Flame Thermoionic Ionization DetectorRPC - Rotation Planar ChromatographyAppendixChromatography and artReferencesAbbreviations usedAcknowledgementsMarket review
Info autore
After working for many years for Heumann Pharma (now part of Pfizer), where she was in particular responsible for the training and education of lab technicians, Elke Hahn-Deinstrop is now a freelance consultant for thin-layer chromatography. She is author of numerous papers and frequent lecturer.
Riassunto
Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition of the practical TLC guide features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced.
Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures.
How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.
Relazione
"...an excellent practical guide to the utilization of this useful analytical procedure." ( Journal of Hazardous Materials , June 25, 2007)
"This impressive book thoroughly discusses a subject not usually covered thoroughly in the analytical chemistry literature." ( CHOICE , June 2007)
