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This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.
Sommario
Identification of Mouse T Follicular Helper Cells by Flow Cytometry.- Identification of Follicular T Helper Cells in Tissue Sections.- Tracking Early T Follicular Helper Cell Differentiation In vivo.- Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination.- Retroviral Vector Expression in TCR Transgenic CD4+ T Cells.- Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells.- In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function.- Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation.- The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses.- Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205.- Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry.- Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells
by Flow Cytometry.- In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses.- Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells.- Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential.- Analysis of Human Blood Memory T Follicular Helper Subsets.- Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood.- Quantifying Helper Cell Function of Human TFH Cells in vitro.
Riassunto
This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.
