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Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.
* Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture
* Features contributions from leading developers and researchers active in 3D cell technology
* Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied
* Includes information on resources and technical support to help initiate the use of 3D culture methods
Sommario
List of contributors, vii
Preface, xi
List of abbreviations, xiv
1 An introduction to the third dimension for routine cell culture, 1
Antonio RomöMorales and Stefan Przyborski Part I: Aggregate¿based technologies 2 Gravity¿enforced microtissue engineering, 23
Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm 3 Physiologically relevant spheroid models for three¿dimensionalcell culture, 50
Nicole A. Slawny and MaryAnn Labant 4 NanoCulture Plate: A scaffold¿based high¿throughput three¿dimensional cell culture system suitable for live imaging and cöculture, 74
Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman 5 Micrömoulded non¿adhesive hydrogels to form multicellular microtissues - the 3D Petri Dish
®, 97
Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan 6 Organotypic microtissues on an air¿liquid interface, 123
Lars E. Sundstrom, Igor Charvet and Luc Stoppini Part II: Hydrogels 7 Materials and assay systems used for three¿dimensional cell culture, 145
Suparna Sanyal and Marshall Kosovsky 8 HyStem
®, a customisable hyaluronan¿based hydrogel matrix for 3D cell culture, 173
T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach¿Carson and D. D. Carson 9 3¿D Life biomimetic hydrogels: A modular system for cell environment design, 197
Brigitte M. Angres and Helmut Wurst Part III: Scaffolds 10 Alvetex
®, a highly porous polystyrene scaffold for routine three¿dimensional cell culture, 225
Antonio RomöMorales, Eleanor Knight and Stefan Przyborski 11 CelluSponge(TM) and Go Matrix as innovative three¿dimensional cell culture platforms, 250
Bramasta Nugraha 12 Mimetix
® electrospun scaffold: An easy¿töuse tool for 3D cell culture in drug discovery and regenerative medicine, 284
Robert J. McKean and Elena Heister Part IV: 3D bioreactor technologies 13 Quasi Vivo
® bioreactor technology, 305
J. Malcolm Wilkinson 14 Three¿dimensional cell¿based assays in hollow fibre bioreactors, 327
John J. S. Cadwell and William G. Whitford 15 Three¿dimensional engineered tissues for high¿throughput compound screening: Mechanical properties of skin and ageing, 351
Michael Conway, Ayla Annac and Tetsuro Wakatsuki 16 Three¿dimensional cell culture in the Rotary Cell Culture System(TM), 370
Stephen S. Navran Glossary, 386
Index, 393
Info autore
Stefan Przyborski is Professor of Cell Technology in the Department of Biosciences, Durham University, UK. He is also the Chief Scientific Officer for ReproCELL Europe, UK. A recognised leader in the field of 3D cell culture, Professor Przyborski has published extensively in this field and presents his work regularly at international events.
Riassunto
Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.
