Receptors - Structure and Function

Klappentext This is a comprehesive review of new methods that can be used for studying cellular receptors and examines how each technique can be used on different receptor types. Zusammenfassung This is a comprehesive review of new methods that can be used for studying cellular receptors and examines how each technique can be used on different receptor types. 1. Probing G protein-coupled receptors by fluorescence spectroscopy; 2. From receptor to endogenous ligand; 3. Point mutations and chimeric receptors in studies of ligand binding domains; 4. Determination of the subunit composition of native and cloned NMDA receptors; 5. Evaluating receptor stoichiometry by fluorescence resonance energy transfer (FRET); 6. Monitoring intracellular trafficking of receptors; 7. Monitoring the turnover kinetics of G protein-coupled receptors; 8. Real time receptor function in vitro: microphysiometry and fluorometric imaging plate reader (FLIPR); 9. Regulation of receptors and receptor-coupled ion channels by protein phosphorylation; 10. Using confocal imaging to measure changes in intracellular ions; 11. Monitoring nuclear receptor function; 12. In vivo gene transfer of antisense to receptors

Dettagli sul prodotto

• Autori: Clare Horton Stanford
• Con la collaborazione di: R. W. Horton (Editore), Roger Horton (Editore), Roger (Department of Pharmacology Horton (Editore), Clare Stanford (Editore), Clare (Department of Pharmacology Stanford (Editore)
• Editore: Oxford University Press
• Lingue: Inglese
• Formato: Tascabile
• Pubblicazione: 29.11.2001
• EAN: 9780199638826
• ISBN: 978-0-19-963882-6
• Pagine: 312
• Serie: Practical Approach Series; Practical Approach (Paperback); Practical Approach (Paperback); Practical Approach
• Categoria: Scienze naturali, medicina, informatica, tecnica > Biologia > Tematiche generali, enciclopedie