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Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on-off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
Sommario
and Reviews.- Calcium-Binding Proteins.- Calcium.- Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation.- The Multifunctional S100 Protein Family.- Ca2+Binding to Proteins Containing ?-Carboxyglutamic Acid Residues.- The Caseins of Milk as Calcium-Binding Proteins.- Calcium-Binding Proteins: Case Studies.- Preparation of Recombinant Plant Calmodulin Isoforms.- Isolation of Recombinant CardiacTroponin C.- Skeletal Muscle Troponin C.- Purification of Recombinant Calbindin D9k.- S100 Proteins.- Cadherins.- ?-Lactalbumin and (Calcium-Binding) Lysozyme.- Recombinant Annexin II Tetramer.- Purification and Characterization of ALG-2 A Novel Apoptosis-Linked Ca2+-Binding Protein.- Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain VI of Calpain.- Neurocalcin.- Crystallization and Structure-Function of Calsequestrin.- Use of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 Domains.- Ca2+-Binding Mode of the C2A-Domain of Synaptotagmin.- Study of Calcineurin Structure by Limited Proteolysis.
Riassunto
Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
