Developmental Hematopoiesis - Methods and Protocols

During the past few decades, technical and conceptual breakthroughs have led to a virtual revolution in developmental biology. In part through cross-species compa- sons and multidisciplinary approaches (combining, for example, classical embry- ogy, genetics, molecular biology, and systems biology), major questions have often been redefined and examined from new angles and with innovative tools. Analyses using such model systems as Drosophila, Xenopus, zebrafish, chick, human, and mouse have underscored the remarkable extent to which molecular and genetic pa- ways are conserved across species and throughout embryonic, fetal, and adult dev- opment. What we learn from the embryo, then, is not only of fundamental interest, but may well have future practical applications in the clinic. A number of excellent volumes, including several in this series (e. g. , Hema- poietic Stem Cell Protocols, Klug and Jordan, eds. , 2002), have surveyed methods used in the study of hematopoiesis-the processes by which the multiple lineages of the blood form from stem and progenitor cells during ontogeny and throughout the entire life of the animal. These collections of protocols have focused largely on the postnatal cells of mouse and human. Our understanding of hematopoietic devel- ment, however, has benefitted enormously from investigations in a variety of org- isms at different stages of ontogeny.

Sommario

Genetic Approaches.- Gene Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse.- Inducible Transgene Expression in Mouse Stem Cells.- Quantitative Trait Analysis in the Investigation of Function and Aging of Hematopoietic Stem Cells.- Analysis of Cell Fate Outcomes in Transplantation Models.- Flow Cytometric Analysis of Hematopoietic Development.- Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells.- Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice.- Animal Models.- Hemocyte Development During Drosophila Embryogenesis.- Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos.- Fate Mapping Hematopoietic Lineages in the Xenopus Embryo.- Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain).- Use of Flow Cytometry and Combined DNA Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo.- Analysis of Hematopoietic Development in the Zebrafish.- Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos.- In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo.- Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development.- Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo.- Hematopoietic Stem Cell Development During Mouse Embryogenesis.- Analysis of Hematopoietic Progenitors in the Mouse Embryo.- In Vivo and In Vitro Assays of Thymic Organogenesis.- Retroviral Transduction in Fetal Thymic Organ Culture.- Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors.- ANew Clonal Assay System for Lymphoid and Myeloid Lineages.- Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture.- Analysis of the Vascular Potential of Hematopoietic Stem Cells.- Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture.- Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation.- Analysis of Hematopoietic Development During Human Embryonic Ontogenesis.- Hematopoietic Development of Human Embryonic Stem Cells in Culture.- Bioinformatics and Functional Genomics.- A Functional Genomics Approach to Hematopoietic Stem Cell Regulation.

Riassunto

During the past few decades, technical and conceptual breakthroughs have led to a virtual revolution in developmental biology. In part through cross-species compa- sons and multidisciplinary approaches (combining, for example, classical embry- ogy, genetics, molecular biology, and systems biology), major questions have often been redefined and examined from new angles and with innovative tools. Analyses using such model systems as Drosophila, Xenopus, zebrafish, chick, human, and mouse have underscored the remarkable extent to which molecular and genetic pa- ways are conserved across species and throughout embryonic, fetal, and adult dev- opment. What we learn from the embryo, then, is not only of fundamental interest, but may well have future practical applications in the clinic. A number of excellent volumes, including several in this series (e. g. , Hema- poietic Stem Cell Protocols, Klug and Jordan, eds. , 2002), have surveyed methods used in the study of hematopoiesis—the processes by which the multiple lineages of the blood form from stem and progenitor cells during ontogeny and throughout the entire life of the animal. These collections of protocols have focused largely on the postnatal cells of mouse and human. Our understanding of hematopoietic devel- ment, however, has benefitted enormously from investigations in a variety of org- isms at different stages of ontogeny.

Dettagli sul prodotto

• Con la collaborazione di: Margaret H. Baron (Editore), Margare H Baron (Editore), Margaret H Baron (Editore)
• Editore: Springer, Berlin
• Lingue: Inglese
• Formato: Tascabile
• Pubblicazione: 26.10.2010
• EAN: 9781617374784
• ISBN: 978-1-61737-478-4
• Pagine: 480
• Dimensioni: 152 mm x 25 mm x 229 mm
• Peso: 689 g
• Illustrazioni: 480 p. 96 illus., 4 illus. in color.
• Serie: Methods in Molecular Medicine; Methods in Molecular Medicine
• Categorie: Scienze naturali, medicina, informatica, tecnica > Medicina > Branche non cliniche; B, Medicine, Hematology, blood;hematopoietic stem cell;stem cells;transplantation