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Informationen zum Autor DAVID L. ANDREWS leads research on fundamental molecular photonics and energy transport, optomechanical forces, and nonlinear optical phenomena. He has over 300 research papers and a dozen of books to his name?including the widely adopted textbook, Lasers in Chemistry. The current focus of his research group is on optical vortices, novel mechanisms for optical nanomanipulation and switching, and light harvesting in nanostructured molecular systems. The group enjoys strong international links, particularly with groups in Canada, Lithuania, New Zealand, and the United States. Andrews is a Fellow of the Royal Society of Chemistry, a Fellow of the Institute of Physics, and a Fellow of SPIE, the international society for optics and photonics. Klappentext Discusses the basic physical principles underlying biomedical photonics, spectroscopy, and microscopy This volume discusses biomedical photonics, spectroscopy, and microscopy, the basic physical principles underlying the technology and its applications. The topics discussed in this volume are: Fluorescence; Single Molecule Detection and Spectroscopy; Resonance Energy Transfer; Biophotonics of Photosynthesis; Optical Sectioning Microscopy and Biological Imaging; Cell Handling and Sorting; Tissue Polarimetry; Optical Waveguide Biosensors; Light Propagation in Highly Scattering Turbid Media; Photodynamic Therapy; Imaging and Probing Cells beyond the Diffraction Limit; and Ophthalmic Technology. Comprehensive and accessible coverage of the whole of modern photonics Emphasizes processes and applications that specifically exploit photon attributes of light Deals with the rapidly advancing area of modern optics Chapters are written by top scientists in their field Written for the graduate-level student in physical sciences; industrial and academic researchers in photonics, graduate students in the area; college lecturers, educators, policymakers, consultants, scientific and technical libraries, government laboratories, NIH. Zusammenfassung Discusses the basic physical principles underlying Biomedical Photonics! spectroscopy and microscopy This volume discusses biomedical photonics! spectroscopy and microscopy! the basic physical principles underlying the technology and its applications. Inhaltsverzeichnis List of Contributors ix Preface xiii 1 Fluorescence 1 David J. S. Birch, Yu Chen, and Olaf J. Rolinski 1.1 Introduction 1 1.2 Spectra 2 1.3 Quantum Yield 6 1.4 Lifetime 12 1.5 Quenching 23 1.6 Anisotropy 30 1.7 Microscopy 38 1.8 Conclusions 48 Acknowledgments 48 References 49 2 Single-Molecule Detection and Spectroscopy 59 Michel Orrit 2.1 Introduction 59 2.2 Experimental Setups 62 2.3 Fluorescence Spectroscopy 66 2.4 Fluorescence Correlation Spectroscopy 72 2.5 Fluorescence Excitation Spectroscopy 78 2.6 Other Detection Methods 86 2.7 Conclusion 93 Acknowledgments 94 References 94 3 Resonance Energy Transfer 101 David L. Andrews, David S. Bradshaw, Rayomond Dinshaw, and Gregory D. Scholes 3.1 Introduction 101 3.2 History of RET 102 3.3 The Photophysics of RET 103 3.4 Investigative Applications of RET in Molecular Biology 113 3.5 The Role of RET in Light-Harvesting Complexes 118 Acknowledgments 122 References 122 4 Biophotonics of Photosynthesis 129 Valter Zazubovich and Ryszard Jankowiak 4.1 Introduction 129 4.2 Structure of Pigment-Protein Complexes and Structure-Function Relationships 130 4.3 Key Concepts in Physics of Pigment-Protein Complexes 133 4.4 Experimental Techniques 141 4.5 Examples 145 4.6 Conclusions 156 Acknowledgments 157
