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The use of human in vitro fertilization in the management of infertility is the outgrowth of years of laboratory observations on in vitro sperm-egg interaction. "The editors of this work have themselves contributed significantly to basic knowledge of the mammalian fertilization process. The observations of Don Wolf on sperm penetration, the block to polyspermy and, most recently, sperm hyperactivation in the monkey and human, Gregory Kopf's elucidation of the mechanisms of sperm activation during penetration and the reciprocal dialogue between sperm and egg, and Barry Bavister's definition of culture conditions and requirements necessary for in vitro oocyte maturation, fertilization and development in model mammalian systems including nonhuman primates have contributed greatly to our understanding of the mammalian fertilization process. Wolf, Kopf and Gerrity have enjoyed substantial interaction with clinicians in Departments of Obstetrics and Gynecology and have been directly involved with successful IVF programs. Both Wolf and Kopf have served as research scientists in the Division of Reproductive Biology at the University of Pennsylvania, which, for more than 22 years, has fostered co-mingling of clinically oriented and basic science faculty. It is through such interaction, which clearly exists at many institutions including the University of Wisconsin, that the process of technology transfer is best served. Without an exquisitely coordinated laboratory, there can be no consistent success in human in vitro fertilization. Quality control is pivotal, but close collaboration between the laboratory and the clinic is also essential as information is shared and correlated.
Sommario
Human IVF-ET: State of the Art 1987.- Selection and Use of Equipment.- Quality Control and Laboratory Monitoring.- Choice, Preparation and Use of Culture Medium.- Mouse Embryo Culture Bioassay.- Preparation and Analysis of Semen Samples.- Assessment of Human Sperm Fertility Potential.- Analysis of Oocyte Quality and Fertilization.- Analysis of Embryonic Development.- Morphology of Human Eggs and Embryos.- Embryo Transfer.- Animal Models for the Study of Fertilization and Early Development in Vitro.- Data Collection and Management.- Gamete Intrafallopian Transfer (GIFT).- Endocrine Measurements.- Cryopreservation of Mammalian Embryos.- Control Mechanisms Regulating Meiotic Maturation of Mammalian Oocytes.- Regulation of Embryonic Development by Environmental Factors.- Clinical Parameters Influencing Success in IVF.- Contributors.
Riassunto
Based on the Second Annual Workshop sponsored by the University of Wisconsin, Extension, August 20-24, 1985
