Nuclear Magnetic Resonance of Biological Macromolecules

Klappentext This volume and its companion, Volume 339 , supplement Volumes 176, 177, 239 , and 261 . Chapters are written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules. Zusammenfassung This volume and its companion! Volume 339 ! supplement Volumes 176! 177! 239 ! and 261 . Chapters are written with a "hands-on" perspective. That is! practical applications with critical evaluations of methodologies and experimental considerations needed to design! execute! and interpret NMR experi Inhaltsverzeichnis Contributors to Volume 338. Preface. Volume in Series. Use of Chemical Shifts in Macromolecular Structure Determination , D.S. Wishart and D.A. Case . Cross-Correlated Relaxation for Measurement of Angles between Tensorial Interactions , H. Schwalbe, T. Carlomagno, M. Hennig, J. Junker, B. Reif, Christian Richter, and C. Griesinger . Applications of Adiabatic Pulses in Biomolecular Nuclear Magnetic Resonance , E. Kupce . Scalar Couplings across Hydrogen Bonds , S. Grzesiek, F. Cordier, and A.J. Dingley . On-Line Cell High-Pressure Nuclear Magnetic Resonance Technique: Application to Protein Studies , K. Akasaka and H. Yamada . Maximum Entropy Reconstruction: Spectrum Analysis and Deconvolution in Multidimensional Nuclear Magnetic Resonance , J.C. Hoch and A.S. Stern . Magnetic Relaxation Dispersion Studies of Biomolecular Solutions , B. Halle and V.P. Denisov . Nuclear Magnetic Resonance-Based Approaches for Lead Generation in Drug Discovery , J.W. Peng, C.A. Lepre, J. Feizo, N. Abdul-Manan, and J.M. Moore . Screening of Compound Libraries for Protein Binding Using Flow-Injection Nuclear Magnetic Spectroscopists , B.J. Stockman, K.A. Farley, and D.T. Angwin . The Internet for Nuclear Magnetic Resonance Spectroscopists , S. Farr-Jones and D. Avizonis . Solid-Phase Synthesis of Selectively Labeled DNA: Applications for Multidimensional Nuclear Magnetic Resonance Spectroscopy , C. Kojima, A.M. Ono, A. Ono, and M. Kainosho . Uniform 13C/15N-Labeling of DNA by Tandem Repeat Amplification , M.H. Werner, V. Gupta, L.J. Lambert, and T. Nagata . 13C Isotopic Enrichment for Nuclear Magnetic Resonance Studies of Carbohydrates and Glycoconjugates , D. Live, L.A. Silks, J. Schmidt . Preparation and Use of 2H-Labeled RNA Oligonucleotides in Nuclear Magnetic Resonance Studies , E.P. Nikonowicz . Investigation of Unusual DNA Motifs , A. Phan, M. Gueron, and J. Leroy . Resonance Assignment and Structure Determination for RNA , J. Cromsigt, B. van Buuren, J. Schleucher, and S. Wijmenga . Solution Nuclear Magnetic Resonance Probing of Cation Binding Sites on Nucleic Acids , J. Feigon, S.E. Butcher, L.D. Finger, and N.V. Hud . Identification and Characterization of Metal Ion Binding Sites in RNA , R.L. Gonzalez and I. Tinoco . Author Index. Subject Index....

Sommario

Contributors to Volume 338.
Preface.
Volume in Series.
Use of Chemical Shifts in Macromolecular Structure Determination, D.S. Wishart and D.A. Case.
Cross-Correlated Relaxation for Measurement of Angles between Tensorial Interactions, H. Schwalbe, T. Carlomagno, M. Hennig, J. Junker, B. Reif, Christian Richter, and C. Griesinger.
Applications of Adiabatic Pulses in Biomolecular Nuclear Magnetic Resonance, E. Kupce.
Scalar Couplings across Hydrogen Bonds, S. Grzesiek, F. Cordier, and A.J. Dingley.
On-Line Cell High-Pressure Nuclear Magnetic Resonance Technique: Application to Protein Studies, K. Akasaka and H. Yamada.
Maximum Entropy Reconstruction: Spectrum Analysis and Deconvolution in Multidimensional Nuclear Magnetic Resonance, J.C. Hoch and A.S. Stern.
Magnetic Relaxation Dispersion Studies of Biomolecular Solutions, B. Halle and V.P. Denisov.
Nuclear Magnetic Resonance-Based Approaches for Lead Generation in Drug Discovery, J.W. Peng, C.A. Lepre, J. Feizo, N. Abdul-Manan, and J.M. Moore.
Screening of Compound Libraries for Protein Binding Using Flow-Injection Nuclear Magnetic Spectroscopists, B.J. Stockman, K.A. Farley, and D.T. Angwin.
The Internet for Nuclear Magnetic Resonance Spectroscopists, S. Farr-Jones and D. Avizonis.
Solid-Phase Synthesis of Selectively Labeled DNA: Applications for Multidimensional Nuclear Magnetic Resonance Spectroscopy, C. Kojima, A.M. Ono, A. Ono, and M. Kainosho.
Uniform 13C/15N-Labeling of DNA by Tandem Repeat Amplification, M.H. Werner, V. Gupta, L.J. Lambert, and T. Nagata.
13C Isotopic Enrichment for Nuclear Magnetic Resonance Studies of Carbohydrates and Glycoconjugates, D. Live, L.A. Silks, J. Schmidt.
Preparation and Use of 2H-Labeled RNA Oligonucleotides in Nuclear Magnetic Resonance Studies, E.P. Nikonowicz.
Investigation of Unusual DNA Motifs, A. Phan, M. Gueron, and J. Leroy.
Resonance Assignment and Structure Determination for RNA, J. Cromsigt, B. van Buuren, J. Schleucher, and S. Wijmenga.
Solution Nuclear Magnetic Resonance Probing of Cation Binding Sites on Nucleic Acids, J. Feigon, S.E. Butcher, L.D. Finger, and N.V. Hud.
Identification and Characterization of Metal Ion Binding Sites in RNA, R.L. Gonzalez and I. Tinoco.
Author Index.
Subject Index.

Dettagli sul prodotto

• Con la collaborazione di: Volker Dotsch (Editore), Thomas L. James (Editore), Uli Schmitz (Editore)
• Editore: ELSEVIER SCIENCE BV
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 12.07.2001
• EAN: 9780121822392
• ISBN: 978-0-12-182239-2
• Pagine: 480
• Serie: Methods in Enzymology; Methods in Enzymology
• Categorie: Scienze naturali, medicina, informatica, tecnica > Biologia > Biochimica, biofisica; SCIENCE / Life Sciences / Biochemistry, biochemistry, Enzymology