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Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.
Sommario
Principles of Flow Cytometry.- Cell Preparation.- Fluorochromes and Fluorescence.- Quality Control in Flow Cytometry.- Experimental Design, Data Analysis, and Fluorescence Quantitation.- Apoptosis Detection by Flow Cytometry.- DNA Analysis by Flow Cytometry.- Immunological Studies of Human Cells.- Calcium: Cytoplasmic, Mitochondria, Endoplasmic Reticulum, and Flux Measurements.- Further Functional Studies.- Cell Sorting by Flow Cytometry.
Relazione
From the reviews:
"This book 'Flow Cytometry: Principles and Applications' focuses on flow cytometry as being an integral part of both basic biological research and clinical diagnosis in pathology. This volume provides a clear and practical manual especially for non-clinicians working in the clinical or experimental laboratory. ... immunologists and haematologists in the field of research, as well as biological researchers working with both human and animal models, will appreciate this book." (Jan Philippé, Acta Clinica Belgica, Vol. 63 (2), 2008)
