Ulteriori informazioni
Informationen zum Autor Dr Anne Bridgen , previously of The University of Ulster is a molecular virologist with extensive research and teaching experience. She was the first scientist to recover infectious virus particles from DNA clones of a segmented RNA virus. Dr Bridgen knows the field and its main players well and has both the knowledge and experience to bring individual expert contributions together around the common theme of reverse genetics. She is currently providing consultancy for a BBSRC grant based at IAH Pirbright. Klappentext Reverse genetics, the manipulation of the DNA of RNA viruses to create a modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells over the past fourteen years. Since most severe viral human and animal pathogens are RNA viruses, including those responsible for polio, hepatitis and influenza, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases.Written by a team of international experts in the field, this book provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. From influenza A virus vaccines, to fluorescent measles virus and quasispecies, this guide explores the progress researchers have made in our current understanding of viral disease and considers where possible applications may lead in the future. Zusammenfassung Reverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. This book provides an account of the developments in reverse genetics of RNA viruses through a range of applications. Inhaltsverzeichnis List of contributors xi Acknowledgements xiii 1 Introduction 1 Anne Bridgen 1.1 Background 1 1.2 Reverse genetics for different classes of genome 2 1.3 Methodology 5 1.4 Difficulties in establishing a reverse genetics system 11 1.5 Recent developments 13 1.6 Are there any boundaries for conducting reverse genetics? 13 References 15 Part I Positive sense RNA viruses 25 2 Coronavirus reverse genetics 27 Maria Armesto, Kirsten Bentley, Erica Bickerton, Sarah Keep and Paul Britton 2.1 The Coronavirinae 27 2.2 Infectious bronchitis 28 2.3 Coronavirus genome organisation 29 2.4 The coronavirus replication cycle 30 2.5 Development of reverse genetics system for coronaviruses including IBV 33 2.6 Reverse genetics system for IBV 37 2.7 Reverse genetics systems for the modification of coronavirus genomes 40 2.8 Using coronavirus reverse genetics systems for gene delivery 49 Acknowledgements 51 References 51 3 Reverse genetic tools to study hepatitis C virus 64 Alexander Ploss 3.1 Introduction: hepatitis C 64 3.2 Hepatitis C virus 65 3.3 Construction of infectious clones for hepatitis C virus 68 3.4 Study of HCV RNA replication in cell culture systems 68 3.5 Use of HCV replicons to study viral replication 70 3.6 Utility of replicons for drug screening 71 3.7 Development of the infectious cell culture systems for HCV 71 3.8 Construction of intergenotypic viral chimeras 72 3.9 Non-JFH1 derived genomes 74 3.10 Cell lines that support HCV replication 74 3.11 Study of HCV in physiologically more relevant cell culture systems 75 3.12 Animal models for HCV infection 76 3.13 Reverse genetics of clinically relevant HCV genotypes in vivo 77 <...
