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Taxonomy is fundamental to understanding the variety of lifeforms, and exciting expansions in molecular biology are re-volutionising the obtained data. This volume reviews the ma-jor molecular biological techniques that are applied in ta-xonomy. The chapters are arranged in three main sections:1)Overviews of important topics in molecular taxonomy; 2) Casestudies of the successful application of molecular methodsto taxonomic and evolutionary questions; 3) Protocols for arange of generally applicable methods. The described techni-ques include DNA-DNA hybridization, DNA fingerprinting, RFLPanalysis, and PCR sequencing.
Sommario
A Prologue.- Past and Future of Taxonomy.- Overviews.- Taxonomy: an Essential Key to Evolutionary Biology.- Variation at the DNA Level: Something for Everyone.- Molecular Systematics at the Species Boundary: Exploiting Conserved and Variable Regions of the Mitochondrial Genome of Animals via Direct Sequencing from Amplified DNA.- Ribosomal RNA Phylogenies.- Evaluating Gene Versus Genome Evolution.- DNA and Higher Plant Systematics: Some Examples From the Legumes.- DNA-DNA Hybridization: Principles and Results.- Satellite DNA.- DNA Fingerprinting.- The Statistical Interpretation of Hypervariable DNAs.- Case Studies.- A Multidimensional Approach to the Evolution and Systematics of Dolichopoda Cave Crickets.- Sperm and Evolution in Drosophila: Morphological and Molecular Aspects.- Episodic Evolutionary Change in Local Populations.- Searching for Speciation Genes in the Species Pair Drosophila Mojavensis and D. Arizonae.- Colonizing Species of Drosophila.- Molecular Taxonomy in the Control of West African Onchocerciasis.- Protocols.- DNA Protocols for Plants.- Prepartion and Visualization of Mitochondrial DNA for RFLP Analysis.- Hybridization of DNA Probes to Filterbound DNA Using the "DIG-DNA Labeling and Detection System Nonradioactive" (Boehringer Mannheim).- DNA Fingerprinting.- The Polymerase Chain Reaction: DNA Extraction and Amplification.- Protocols for 4-Cutter Blots and PCR Sequencing.- Ribosomal RNA Sequencing.- Quantitative DNA:DNA Hybridization and Hydroxyapatite Elution.- A Protocol for the TEACL Method of DNA-DNA Hybridization.- List of Participants.
