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A better "casting" could not be conceived. The authors of this book are gold smiths on the subject. I have followed their work since their "entry" into cyto genetics and I have a high esteem for them. I consider it an honour to be asked to write the preface of their opus. Paul Popescu, Directeur de Recherche at INRA, has also played a promi nent part in the development of animal cytogenetics, especially in domestic animals. He is able to tell you the cost of a translocation in a pig breeding farm or a cow population: a fortune! P. Popescu has played a great part in gene mapping of these species using "in situ DNA hybridisation". His contributions are recognised world-wide. His laboratory receives many visitors every year and it serves as a reference for domestic animal cytogenetics. Helene Hayes, Charge de Recherche at INRA, has collaborated with P. POPESCU in the elaboration of the "at hand" techniques and in many other discoveries which are listed in her bibliography. She showed the fascinating correspondence between bovine and human chromosomes and the com pared gene maps of domestic bovidae.
Sommario
I Preparation of Chromosome Spreads.- I.1 Cell culture techniques.- I.2 Preparation of chromosomes in prophase or prometaphase.- I.3 Direct techniques and very short term development cultures.- I.4 Preparation of chromosome spreads.- I.5 Staining techniques of chromosome spreads.- II Chromosome Banding Techniques.- II. 1 Introduction.- II.2 Techniques based on DNA structure.- II.3 Banding techniques based on DNA replication.- II.4 Techniques of chromosome differentiation based on DNA base composition.- II.5 Heterochromatin staining.- II.6 Staining of nucleolar organiser regions NOR.- II.7 Techniques of sequential banding.- III In Situ Hybridisation Techniques.- III.1 Introduction.- III.2 Methods.- III.3 Remarks on other applications of in situ hybridization.- IV Methods of Germ Cells Study.- IV.1 Meiosis in male.- IV.2 Meiosis in the mammalian female.- IV.3 Study of the spermatozoa by interspecific in vitro fertilisation (insemination).- V The Lampbrush Chromosomes of Amphibians.- V.1Introduction: The basis of lampbrush chromosomes mapping.- V.2 Technique for the preparation of lampbrush chromosomes for light microscopy.- V.3 Preparation of lampbrush chromosomes for electron microscopy.- V.4 Preparation of mitotic chromosomes.- V.5 Analysis of lampbrush chromosomes.- V.6 The importance of the lampbrush chromosomes.- VI Techniques for the study of Drosophila Chromosomes.- VI.1 Mitotic chromosomes.- VI.2 Polythene chromosomes.- VII Techniques for the stuey of Interphase Nucleus.- VII.1 Sex chromatin examination.- VII.2 Released chromatin (Chromatin halo).- VII.3 In situ hybridisation of interphasic nuclei.- VIII Application of Flow Cytometry and Slit-Scan Fluorometry in Analysis and Sorting of Mammalian Chromosomes.- VIII.1 Introduction.- VIII.2 Principles of the flow cytometry.- VIII.3 Methods of chromosome preparation and staining.- VIII.4 Measurements and evaluation in flow cytometry.- VIII.5 In situ hybridisation to chromosomes in suspension.- S Solutions for chromosome staining and banding techniques.- M Miscellaneous.- L Solutions for lampbrush chromosomes.- F Solutions for flow and slit scan cytometry.- H Solutions for in situ hybridization.- CM Culture media.- SS Stock solutions.- B Buffer solutions.- V Solutions for in vivo treatments.- References.
